Study on Polysaccharide by the Fluorescence Method. I. Fluorescence Depolarization of the Fluorescein Conjugates of Amylose and Dextran

Abstract

Amylose and dextran conjugated with fluorescein were prepared and the degree of fluorescence polarization of these conjugates was measured with changing solvent viscosity at constant temperature (25°C). The results obtained were analyzed by Perrin’s equation to calculate the mean rotational relaxation time. The value for dextran conjugate was very small compared with that for amylose. This suggests that the molecular chain of dextran is more flexible than that of amylose. Polarizations of these conjugates gradually departed from the Perrin equation when the temperature was raised in a given solvent. This discrepancy can be attributed to an accelerated loosening of the internal structure of these polysaccharide chains. Thus it seems that amylose and dextran molecules below the transition temperatures have partly ordered conformations with restricted rotation about glycosidic linkage.

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Kitamura, S., Yunokawa, H. & Kuge, T. Study on Polysaccharide by the Fluorescence Method. I. Fluorescence Depolarization of the Fluorescein Conjugates of Amylose and Dextran. Polym J 14, 85–91 (1982). https://doi.org/10.1295/polymj.14.85

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Keywords

  • Polysaccharide
  • Amylose
  • Dextran
  • Fluorescence Polarization
  • Fluorescein Conjugate
  • Segmental Motion
  • Flexibility
  • Conformational Transition

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