Abstract
13C NMR parameters such as chemical shift, spin–lattice relaxation time (T1), and nuclear Overhauser enhancement (NOE) were measured for the arginine residue of clupeine in aqueous solution. The correlation time for molecular reorientation of the backbone and side-chain carbons were estimated from T1 and NOE. The correlation time for the α-carbon in the backbone was 0.57 ns at 300 K, indicating rapid segmental motion and that the clupeine molecule is thus flexible. The side chain carbons are more mobile due to the presence of internal motion than the backbone carbon. The internal motion of the side chain of arginine was found to be more restricted compared with that of lysine. This is interpreted in terms of the presence of the bulky guanidino group.
Similar content being viewed by others
Article PDF
References
T. Ando and K. Suzuki, Biochem. Biophys. Acta, 140, 375 (1967).
K. Suzuki and T. Ando, J. Biochem. (Tokyo), 63, 403 (1968).
C. Toniolo, G. M. Bonora, F. Marchiori, G. Borin, and F. Filippi, Biochem. Biophys. Acta, 576, 429 (1979).
E. M. Bradbury, C. Crane-Robinson, H. Goldman, H. W. E. Rattle, and R. M. Stephens, J. Mol. Biol., 29, 507 (1967).
T. Hiraoki, A. Tsutsumi, and K. Hikichi, Rep. Prog. Polym. Phys. Jpn., 20, 675 (1977).
G. M. Bonora, L. Ferara, L. Paolillo, C. Toniolo, and E. Trivellone, Eur. J. Biochem., 93, 13 (1979).
W. Horsley, H. Sternlicht, and J. S. Cohen, J. Am. Chem. Soc., 92, 680 (1970).
H. Saito, T. Ohki, M. Kodama, and C. Nagata, Biopolymers, 17, 2587 (1978).
D. Doddrell, V. Glushko, and A. Allerhand, J. Chem. Phys., 56, 3683 (1972).
F. P. Ottensmeyer, R. F. Whiting, and A. P. Korn, Proc. Natl. Acad. Sci., U.S.A., 72, 4953 (1975).
A. Allerhand and E. Oldfield, Biochemistry, 12, 3428 (1973).
H. Saito and I. C. P. Smith, Arch. Biochem. Biophys., 158, 154 (1973).
D. A. Torchia and J. R. Lyerla, Biopolymers, 13, 97 (1974).
S. Sase, Y. Suzuki, Y. Inoue, and R. Chûjô, Biopolymers, 16, 95 (1977).
A. Tsutsumi, B. Perly, A. Forchioni, and C. Chachaty, Macromolecules, 11, 977 (1978).
T. Hiraoki and K. Hikichi, Polym. J., 11, 299 (1979).
N. Higuchi, T. Hiraoki, and K. Hikichi, Macromolecules, 13, (1980) in press.
D. E. Woessner, J. Chem. Phys., 37, 647 (1962).
τint values for each polymers were calculated from T1 date cited in references 8, 16, and 17.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hiraoki, T., Hikichi, K. 13C Magnetic Relaxation Study of Hindered Motion of Arginine in Clupeine. Polym J 12, 25–28 (1980). https://doi.org/10.1295/polymj.12.25
Issue Date:
DOI: https://doi.org/10.1295/polymj.12.25