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miR-148a is an androgen-responsive microRNA that promotes LNCaP prostate cell growth by repressing its target CAND1 expression

Prostate Cancer and Prostatic Diseases volume 13, pages 356–361 (2010)Cite this article

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Abstract

Recent advances in cancer biology reveal that microRNAs (miRNAs) are involved in the regulation of cancer-related genes, or they function as tumor suppressors or oncogenes. In prostate cancer, evidence has accumulated for the contribution of the androgen-dependent gene network to tumor growth, although the precise functions of miRNAs in prostate cancer remain to be investigated. Here, we identified androgen-responsive miRNAs by the short RNA sequencing analysis in LNCaP prostate cancer cells. Among 10 miRNAs with known sequences, we have determined that miR-148a reduces the expression of cullin-associated and neddylation-dissociated 1 (CAND1), a negative regulator of SKP1-Cullin1-F-box (SCF) ubiquitin ligases, by binding to the 3′-untranslated region of CAND1 mRNA. CAND1 knockdown by small interfering RNA promoted the proliferation of LNCaP cells. Our study indicates the potential contribution of miR-148a to the growth of human prostate cancer.

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Acknowledgements

We are grateful to Chihiro Sakaba, Hiromi Sano, Ryoko Ishihara, and Kazumi Yamaguchi (Riken) for their assistance. This work was supported by Grants of the Genome Network Project, Cell Innovation Program, and the DECODE from the MEXT; by the Program for Promotion of Fundamental Studies in Health Science of the NIBIO; by Grants from the Japan Society for the Promotion of Science; by Grants-in-Aid from the Ministry of Health, Labor and Welfare, and the Promotion and Mutual Aid Corporation for Private School of Japan.

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Authors and Affiliations

  1. Department of Anti-Aging Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan

    T Murata, K Takayama, T Urano & S Inoue

  2. Department of Geriatric Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan

    T Murata, K Takayama, T Urano, Y Ouchi & S Inoue

  3. Riken Omics Science Center, RIKEN Yokohama Institute, Kanagawa, Japan

    S Katayama, C Kawazu, A Hasegawa & Y Hayashizaki

  4. Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Hidaka-shi, Saitama, Japan

    T Urano, K Horie-Inoue, K Ikeda & S Inoue

  5. Department of Urology, Faculty of Medicine, The Nihon University, Itabashi-ku, Tokyo, Japan

    S Takahashi

  6. Department of Urology, Graduate School of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan

    Y Homma

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  1. T Murata
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Correspondence to S Inoue.

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Murata, T., Takayama, K., Katayama, S. et al. miR-148a is an androgen-responsive microRNA that promotes LNCaP prostate cell growth by repressing its target CAND1 expression. Prostate Cancer Prostatic Dis 13, 356–361 (2010). https://doi.org/10.1038/pcan.2010.32

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  • DOI: https://doi.org/10.1038/pcan.2010.32

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