Figure 6

From: FAM49B, a novel regulator of mitochondrial function and integrity that suppresses tumor metastasis

Figure 6

FAM49B expression alters mitochondrial function and cell redox states. (a) ROS levels (upper panels) and mitochondrial SOD activity levels (lower panels) were evaluated in shCTRL and shFAM49B CFPAC1, T3M4 and HPDE cells. (b, c) GSH and NADPH oxidase activity levels in shCTRL and shFAM49B CFPAC1, T3M4 and HPDE cells. (d) Analysis of mitochondrial respiratory chain complex I-IV activity levels in shCTRL and shFAM49B CFPAC1 and T3M4 cells, expressed as nmol NAD+/min/mg mitochondrial protein for complex I, nmol Cyt c reduced/min/mg mitochondrial protein for complexes II–III and nmol Cyt c oxidized/min/mg mitochondrial protein for complex IV. (e) shCTRL and shFAM49B CFPAC1, T3M4 and HPDE cell oxygen consumption rates (OCRs). Antimycin was used as a positive control for PCR inhibition. (f) Relative ATP levels in shCTRL and shFAM49B CFPAC1, T3M4 and HPDE cells. All the graphs illustrate the mean results of three independent experiments±s.e.m. *P<0.05, **P<0.01*** and P<0.001 values indicate significant differences from shCTRL.