Figure 5

From: FAM49B, a novel regulator of mitochondrial function and integrity that suppresses tumor metastasis

Figure 5

FAM49B regulates mitochondrial dynamics. (a) MitoTracker staining was used to analyze mitochondrial morphology in CFPAC1, T3M4 and HPDE cells. The cells were analyzed with a laser-scanning microscope (Zeiss LSM5 Pascal). (b) Quantitation of the mitochondrial morphologies observed in the cells described in a, n>50 cells, was blindly performed by three people in three independent experiments. The bars represent the mean percentages of the mixed (black bars), tubular (gray bars) or fragmented (red bars) mitochondrial phenotypes from one representative experiment. (c) CFPAC1, T3M4 and HPDE cell lysates were analyzed for mitochondrial dynamics regulator expression by western blotting with anti-FAM49B, anti-DRP1, anti-MFN1, and anti-MFN2 Abs. Actin was used as a loading control.