Figure 4

From: FAM49B, a novel regulator of mitochondrial function and integrity that suppresses tumor metastasis

Figure 4

Localization of FAM49B in the mitochondria. (a) Cells co-stained with Mitotracker (red) and anti-FAM49B antibody (green) was detected by immunofluorescence. Tissue sections were analyzed with a laser scanning confocal microscope (Zeiss LSM5 Pascal, Jena, Germany) using its multichannel acquisition mode to avoid fluorescence cross talk. Images were acquired with a × 100 oil-immersion objective, scale bar 10 μm. The pixels indicating co-localization are shown in yellow. (b) FAM49B expression in the cytosolic (Cyto) and mitochondria-enriched (Mito) fractions of HPDE, CFPAC1 and T3M4 cells was measured by western blot analysis. The mitochondrial marker ATP5A was used as a control of the quality of the mitochondrial fractions. GAPDH served as a cytosolic marker.