Figure 3 | Oncogene

Figure 3

From: Disrupting G6PD-mediated Redox homeostasis enhances chemosensitivity in colorectal cancer

Figure 3

Knockdown of G6PD enhances oxaliplatin-induced apoptosis in CRC cells. (a) Increased ROS levels in CRC cells treated with oxaliplatin (20 μm or 40 μm) for 6 h. (b) Immunoblotting analysis of G6PD, Nrf2, Lamin A and β-Actin in DLD-1 and HCT116 cells treated with oxaliplatin or transfected with Nrf2 siRNA for 48 h. (c) qPCR analysis of G6PD expression in DLD-1 and HCT116 cells treated with oxaliplatin or transfected with Nrf2 siRNA for 48 h. (d) Increased ROS levels in G6PD-knockdown cells compared with control cells treated with oxaliplatin (40 μm) for 24 h, and representative histograms are shown. (e) The cell viability were measured using MTS assays in G6PD-knockdown and control cells treated with oxaliplatin for 48 h. (f) Cell apoptosis was measured by Annexin-V/PI assay in G6PD-knockdown and control cells treated with oxaliplatin (40 μm) for the indicated period of time. Numbers indicate percentages of live cells. Data are presented as the mean±s.d. (n=3). **P<0.01 for indicated comparison (Student unpaired t-test).

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