Figure 5

From: Detecting intratumoral heterogeneity of EGFR activity by liposome-based in vivo transfection of a fluorescent biosensor

Figure 5

Effect of Mo-IPQA on EGFR activity in HCC1954 xenograft tumours monitored with Picchu-X biosensor. (a) FRET/FLIM images of Picchu-X biosensor expressed in untreated HCC1954 tumour cells (animal were injected with lipopolyplexes modified with Y-I peptide and peptide/DNA in a 2:1 ratio) and lifetime distribution among them. (b) Representative images of Picchu-X biosensor expressing cells from untreated and treated animals with Mo-IPQA (200-μg dose). (c) Quantification of lifetime changes in tumour cells after treatment of animals with Mo-IPQA at 2-μg and 200-μg doses. Data were expressed as means±s.e.m. (*P<0.005, **P<0.0001, Student’s t-test, tumours from four animals per group were imaged). (d) The association between proximity of the nearest macrophage to tumour cells and EGFR activity was evaluated by ANCOVA; in control and high-dose treated groups. (e) The association between proximity of the nearest CD31+ blood vessels to tumour cells and EGFR activity (P values presented for both ANCOVA models).