Figure 3

From: Detecting intratumoral heterogeneity of EGFR activity by liposome-based in vivo transfection of a fluorescent biosensor

Figure 3

Optimization of lipopolyplexes to maximize transfection and expression of Picchu-X biosensor. (a) Transfection efficiency of lipopolyplexes modified with different targeting peptides in HCC1954 cells (same as in Figure 2c). (b) Expression of Picchu-X biosensor in the same cells treated in (a). (c, d). Transfection and Picchu-X biosensor expression in HCC1954 cells treated with lipopolyplexes modified with Y-I peptide using different peptide/DNA ratios. One-way ANOVA to test differences between group means. P values are derived from a post-hoc Tukey’s test subsequently performed to compare means of individual groups while controlling for family-wise error rate. The difference was statistically significant between control and treatment groups, and data were expressed as means±s.e.m. (*P<0.01, N=3).