LZTFL1 is expressed in normal lung epithelium and downregulated in NSCLCs. (a) LZTFL1 expression in human normal lung epithelium and lung cancers. Clinically annotated, paired normal lung and NSCLC tumor specimens were used to stain LZTFL1 by IHC. Six representative pairs are shown. LZTFL1 is expressed specifically in the cytoplasm of differentiated airway epithelial cells but not in basal cells (arrows). LZTFL1 is downregulated in adeno and squamous cell carcinoma. Interestingly, a membranous and nuclear staining of LZTFL1 can be seen in some lung cancers (arrow head). Scale bar, 40 μM. (b) Cytoplasmic staining of LZTFL1 in adenocarcinoma from non-recurrent (n=13) and recurrent (n=8) patients were scored and subjected to statistical analysis (Pearson r= −0.788, *P<0.01). (c, d) Kaplan–Meier survival plot of lung adenocarcinoma (c, n=719) and never-smoking lung adenocarcinoma patients (d, n=143) using mRNAs of LZTFL1 from an aggregate mega-database and software kmplot. (e, f) Kaplan–Meier survival plot of lung adenocarcinoma patients using LZTFL1 mRNA (e, n=482) and CNV segment mean (f, n=476) from TCGA level 3 data.