Figure 3 | Oncogene

Figure 3

From: RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription

Figure 3

RMP knockdown reduced characteristics of liver T-ICs. (a) Spheroid assay of CSQT-2 and MHCCLM3 cells was performed, and the mRNA of spheroids was extracted and subjected to real-time PCR. Error bars represent s.e.m. from at least three independent experiments. *P<0.05 (Student’s t-test). (b) Spheroid assay of CSQT-2 control/CSQT-2 shRMP and MHCCLM3 control/shRMP cells were performed. Bars, 500 μm Data are mean±s.e.m. and are representative of three independent experiments. *P<0.05; **P<0.01 (Student’s t-test). (c) The mRNA expression of genes related to stemness in CSQT-2 control/shRMP and MHCCLM3 control/shRMP cells by real-time reverse transcriptase (RT)–PCR. Data are mean±s.e.m. and are representative of three independent experiments. *P<0.05; **P<0.01; ***P<0.001 (Student’s t-test). (d) The mRNA expression of genes related to hepatic T-ICs biomarkers in CSQT-2 control/shRMP and MHCCLM3 control/shRMP cells by real-time RT–PCR. Data are mean±s.e.m. and are representative of three independent experiments. *P<0.05; **P<0.01 (Student’s t-test). (e) The mRNA expression of genes related to EMT in CSQT-2 control/shRMP and MHCCLM3 control/shRMP cells by real-time RT–PCR. Data are mean±s.e.m. and are representative of three independent experiments. *P<0.05; **P<0.01; ***P<0.001 (Student’s t-test). (f) Knockdown of RMP in CSQT-2 and MHCCLM3 cells resulted in upregulation of E-cadherin as determined by immunoblot.

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