RMP expression was associated with venous metastases in HCC. (a, b) Expression of RMP in human HCC cell lines was examined by western blot assay and real-time PCR. Error bars represent s.e.m. from at least three independent experiments. (c) Real-time PCR analysis to quantify the endogenous levels of RMP in 40 HCC patients with PVTT. (PVTT, PVTT tumor tissues; tumor, HCC tumor tissues; para-tumor, adjacent normal-like tissue). Data are mean±s.e.m. and are representative of three independent experiments. *P<0.05; **P<0.01 (Student’s t-test). (d) Representative images of RMP staining in PVTT, HCC tumor and para-tumor. Bars, 100μm (upper panel). Statistics of the RMP immunohistochemistry (IHC) scores. P<0.0001 (Chi-square test) (low panel).