Figure 2 | Oncogene

Figure 2

From: Tumor hypoxia induces nuclear paraspeckle formation through HIF-2α dependent transcriptional activation of NEAT1 leading to cancer cell survival

Figure 2

NEAT1 is induced by hypoxia in solid tumors. qPCR analysis of NEAT1 expression in (a) MCF-7, (b) MDA-MB-231 and (c) MDA-MB-468 tumor xenografts treated with the anti-angiogenesis agent, bevacizumab, or vehicle only showing increased NEAT1 levels following treatment with bevacizumab. Three independent experiments were analyzed on a minimum of 100 cells per replicate. (d) H&E staining, (e) immunohistochemistry for CA9 and (f) RNA-fluorescent in situ hybridization analysis for NEAT1 (red channel=NEAT1, blue channel=DAPI counterstain) in MDA-MB-231 tumor xenografts showing co-localization of NEAT1 and CA9 expression in a penumbra distant from a blood vessel.

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