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Heterogeneity in ERK activity as visualized by in vivo FRET imaging of mammary tumor cells developed in MMTV-Neu mice

Oncogene volume 34, pages 1051–1057 (2015)Cite this article

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Abstract

Human epidermal growth factor receptor2/Neu, which is overexpressed in about 30% of human breast cancers, transduces growth signals in large part via the Ras–Raf–MEK–ERK pathway. Nevertheless, it is a matter of controversy whether high ERK activity in breast cancer tissues correlates with better or worse prognosis, leaving the role of ERK activity in the progression of breast cancers unresolved. To address this issue, we live-imaged ERK activity in mammary tumors developed in mouse mammary tumor virus-Neu transgenic mice, which had been crossed with transgenic mice expressing a Förster resonance energy transfer biosensor for ERK. Observation of the tumor by two-photon microscopy revealed significant heterogeneity in ERK activity among the mammary tumor cells. The level of ERK activity in each cell was stable up to several hours, implying a robust mechanism that maintained the ERK activity within a limited range. By sorting the mammary tumor cells on the basis of their ERK activity, we found that ERKhigh cells less efficiently generated tumorspheres in vitro and tumors in vivo than did ERKlow cells. In agreement with this finding, the expressions of the cancer stem cell markers CD49f, CD24 and CD61 were decreased in ERKhigh cells. These observations suggest that high ERK activity may suppress the self-renewal of mammary cancer stem cells.

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Acknowledgements

J3B1 cells were kindly provided by Roberto Montesano. We thank Y Inaoka, K Hirano, K Takakura, R Tabata, Y Kitagawa and A Kawagishi for their technical assistance. We are grateful to the members of the Matsuda Laboratory for their helpful discussions. MM was supported by a Grant-in-Aid for Scientific Research on the Innovative Area of ‘Fluorescence Live imaging’ (No. 22113002) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), and by an Innovative Techno-Hub for the Integrated Medical Bio-imaging Project of the Special Coordination Funds for Promoting Science and Technology by MEXT, Japan. YK was supported by a Grant-in-Aid for JSPS Fellows from MEXT, Japan. The animal protocols were reviewed and approved by the Animal Care and Use Committee of Kyoto University Graduate School of Medicine (No. 10584).

Author information

Authors and Affiliations

  1. Department of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University, Kyoto, Japan,

    Y Kumagai & M Matsuda

  2. Innovative Techno-Hub for Integrated Medical Bio-Imaging, Kyoto University, Kyoto, Japan

    H Naoki

  3. Life & Industrial Products Development Department 1, R&D Division, Olympus Corporation, Hachioji, Japan

    E Nakasyo

  4. Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto, Japan

    Y Kamioka & M Matsuda

  5. Imaging Platform for Spatio-Temporal Information, Graduate School of Medicine, Kyoto University, Kyoto, Japan

    Y Kamioka

  6. Department of Oncologic Pathology, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Kahoku-gun, Ishikawa, Japan

    E Kiyokawa

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  1. Y Kumagai
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Correspondence to M Matsuda.

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Kumagai, Y., Naoki, H., Nakasyo, E. et al. Heterogeneity in ERK activity as visualized by in vivo FRET imaging of mammary tumor cells developed in MMTV-Neu mice. Oncogene 34, 1051–1057 (2015). https://doi.org/10.1038/onc.2014.28

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