Figure 4 | Oncogene

Figure 4

From: p53 deficiency enhances mitotic arrest and slippage induced by pharmacological inhibition of Aurora kinases

Figure 4

p53 is activated after inhibition of AURKA and AURKB. (a) Activation of p53 by Alisertib. HCT116 cells were incubated with the indicated concentrations of Alisertib for 24 h. Lysates were prepared and analyzed with immunoblotting. Uniform loading was confirmed by immunoblotting for actin. (b) p53-dependent activation of p21CIP1/WAF1 by Alisertib. HepG2, HCT116 and HCT116(p53−/−) cells were incubated with buffer or Alisertib (250 nM or 1 μM) for 24 h. Lysates were prepared and analyzed with immunoblotting. (c) Activation of p53-p21CIP1/WAF1 pathway by inhibitors of AURKA and AURKB. HCT116 cells were treated with buffer, Barasertib (25 nM and 50 nM) or MK-5108 (0.5 μM and 1 μM) for 24 h. Lysates were prepared and analyzed with immunoblotting.

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