Figure 7

From: Desmoglein 3 promotes cancer cell migration and invasion by regulating activator protein 1 and protein kinase C-dependent-Ezrin activation

Figure 7

Overexpression of Dsg3 enhances the c-Jun S63 phosphorylation and activates the AP-1 transcriptional activity. (a) Human phosphokinase array data (part) presented as the log 2 ratio of fold increase in A431-D3 against Vect cells. Significant increase in the phosphorylation of c-Jun S63 was observed among other kinases. (b) Dsg3 enhanced the AP-1 activity assessed by the luciferase assay. AP-1 reporter or Vect plasmid was transfected into A431 parental cells together with various concentrations of pBABE-hDsg3.myc topped up to 1 μg plasmid(s) with pBABE-GFP. A clear dose-dependent increase of luciferase activity was consistently seen in cells with overexpression of Dsg3 (experiment n>3, **P<0.01, ***P<0.001). (c) Dsg3 silencing in A431 reduced the luciferase activity more than twofold compared with scrambled small interfering RNA-treated cells (**P<0.01).