Figure 4

From: Desmoglein 3 promotes cancer cell migration and invasion by regulating activator protein 1 and protein kinase C-dependent-Ezrin activation

Figure 4

Dsg3 is required for the localization and activation of Ezrin at the plasma membrane in A431 cells. (a) Confocal images of A431-Vect and -C7 with high levels of Dsg3 showed strong colocalization between Dsg3 (purple), the peripheral pERM (red) and cortical F-actin bundles (green), and its correlation with cell contraction and stratification. Arrows indicate the central stratified rounded cells in the colony that express high levels of Dsg3 coupled with strong cortical actin bundles and the pERM expression. These changes however appeared to be missing in A431-Vect cells in which Dsg3 expression remained low and little pERM signal was seen. The quantitation for each channel is presented on the right (mean±s.e.m., ***P<0.001). (b) Colocalization of the indicated proteins in A431 cells with or without Dsg3 knockdown (KD). Confocal images were obtained in six arbitrary fields from each coverslip. Percentage of colocalization of the indicated proteins was determine by ImageJ (highlighted in white) and is presented as mean±s.d. (*P<0.05, ***P<0.001). Scale bars, 20 μm.