Figure 2

From: Desmoglein 3 promotes cancer cell migration and invasion by regulating activator protein 1 and protein kinase C-dependent-Ezrin activation

Figure 2

Dsg3 promotes cell migration and invasion. (a) Transwell cell migration assay of A431 (including polyclonal and monoclonal cells) and SqCC/Y1 cells showed that overexpression of Dsg3 enhanced cell migration compared with Vect cells. HaCaT (HaC) normal keratinocytes served as a negative control in this experiment. Data of A431 were pooled from three independent experiments (n=9, mean±s.e.m.). (b) Transwell cell invasion assays of SqCC/Y1 cells showed that overexpression of Dsg3 (both polyclonal and monoclonal cells) promoted cell invasion compared with control cells (mean±s.d., *P<0.05, **P<0.01, ***P<0.001). (c) Hematoxylin and eosin staining of 2-week-organotypic culture of SqCC/Y1 cells shows augmented cell invasion in SqCC/Y1 with overexpression of Dsg3 (Sq-D3; red arrow in Sq-D3). The middle panels are the representative binary images and the epithelial compartment is highlighted in red that enable better visualization of cell invasion. The lower panels are immunohistochemistry for myc-tag staining demonstrating positive staining of myc-tag in hDsg3.myc-transducing cells, but only weak nucleus staining (endogenous c-Myc) in control. (d) Quantitation of cell invasion from two independent experiments and the invaded epithelial cell clusters scored for the parameters are shown (n=13, mean±s.d., *P<0.05, ***P<0.001). (e) Cell invasion index80 indicated a significant increase in Sq-D3 cells compared with control cells (mean±s.e.m.,***P<0.001). Scale bars, 50 μm.