Figure 1

From: Desmoglein 3 promotes cancer cell migration and invasion by regulating activator protein 1 and protein kinase C-dependent-Ezrin activation

Figure 1

Overexpression of Dsg3 induces filopodia formations in A431 cells. (a) Fluorescent confocal images of Vect, polyclonal (D3) and monoclonal (C2, C7) cells labeled with mouse Ezrin antibody. Pronounced membrane protrusions were particularly seen in Dsg3-overexpressing cells. The bar chart on the right shows quantitation of the percentage of cells with membrane projections covering >50% of cell periphery in the field (experiment n>3, 100 cells were analyzed from each group and are presented as mean±s.d., *P<0.05, ***P<0.001). (b) The enhanced membrane projections in A431-D3 cells ((i) epi-fluorescent microscopic image, inset: confocal image) were largely inhibited by Dsg3 knockdown (KD; arrows in (ii) and (iii)). Note that the collapse of membrane projections was coupled with disruption of E-cadherin adhesion in knock-down cells compared with control. The enlarged images of boxed areas in (ii) are displayed on the right. Scram, scrambled small interfering RNA control. (c) Western blotting of Dsg3 KD in A431-Vect and -D3 cells. Scale bars, 20 μm.