Figure 2 | Oncogene

Figure 2

From: The aryl hydrocarbon receptor regulates focal adhesion sites through a non-genomic FAK/Src pathway

Figure 2

TCDD triggers the early phosphorylation and activation of FAK/Src through an AhR-dependant pathway. (a and b) HepG2 cells were treated for increasing lengths of time (0,1 and 2 h) with TCDD (25 nM). Western blots were performed with cells extracts using (a) antibodies to: FAK total, FAK pY397, FAK pY861 or FAK pY925; (b) antibodies to: pan-Src or Src-pY416. The right panel shows the quantification of three independent experiments. (c) HepG2 cells were treated with TCDD (25 nM) and/or aNF (5 μM) for 24 h. Western blots were performed with cell extracts using antibodies to: FAK total, FAK pY397, FAK pY861 or FAK pY925. The right panel shows the quantification of three independent experiments. (d) HepG2 cells were treated with TCDD (25 nM) and/or aNF (5 μM) for 24 h. Western blots were performed with cell extracts using antibodies against pan-Src or Src-pY416. The right panel shows the quantification of three independent experiments. **P<0.01 and *P<0.05 as compared with controls. A full colour version of this figure is available at the Oncogene journal online.

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