Abstract
Although numerous mouse models of B-cell malignancy have been developed via the enforced expression of defined oncogenic lesions, the feasibility of generating lineage-defined human B-cell malignancies using mice reconstituted with modified human hematopoietic stem cells (HSCs) remains unclear. In fact, whether human cells can be transformed as readily as murine cells by simple oncogene combinations is a subject of considerable debate. Here, we describe the development of humanized mouse model of MYC/BCL2-driven ‘double-hit’ lymphoma. By engrafting human HSCs transduced with the oncogene combination into immunodeficient mice, we generate a fatal B malignancy with complete penetrance. This humanized-MYC/BCL2-model (hMB) accurately recapitulates the histopathological and clinical aspects of steroid-, chemotherapy- and rituximab-resistant human ‘double-hit’ lymphomas that involve the MYC and BCL2 loci. Notably, this model can serve as a platform for the evaluation of antibody-based therapeutics. As a proof of principle, we used this model to show that the anti-CD52 antibody alemtuzumab effectively eliminates lymphoma cells from the spleen, liver and peripheral blood, but not from the brain. The hMB humanized mouse model underscores the synergy of MYC and BCL2 in ‘double-hit’ lymphomas in human patients. Additionally, our findings highlight the utility of humanized mouse models in interrogating therapeutic approaches, particularly human-specific monoclonal antibodies.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Aukema SM, Siebert R, Schuuring E, van Imhoff GW, Kluin-Nelemans HC, Boerma EJ et al. Double-hit B-cell lymphomas. Blood, 2011; 117: 2319–2331.
Snuderl M, Kolman OK, Chen YB, Hsu JJ, Ackerman AM, Dal Cin P et al. B-cell lymphomas with concurrent IGH-BCL2 and MYC rearrangements are aggressive neoplasms with clinical and pathologic features distinct from Burkitt lymphoma and diffuse large B-cell lymphoma. Am J Surg Pathol, 2010; 34: 327–340.
Hasserjian RP, Ott G, Elenitoba-Johnson KS, Balague-Ponz O, de Jong D, de Leval L Commentary on the WHO classification of tumors of lymphoid tissues (2008): ‘Gray zone’ lymphomas overlapping with Burkitt lymphoma or classical Hodgkin lympoma. J Hematopathol, 2009; 2: 89–95.
Bacher U, Haferlach T, Alpermann T, Kern W, Schnittger S, Haferlach C Several lymphoma-specific genetic events in parallel can be found in mature B-cell neoplasms. Genes Chromosomes Cancer, 2011; 50: 43–50.
Tomita N BCL2 and MYC dual-hit lymphoma/leukemia. J Clin Experiment Hematopathol, 2011; 51: 7–12.
Li S, Lin P, Fayad LE, Lennon PA, Miranda RN, Yin CC et al. B-cell lymphomas with MYC/8q24 rearrangements and IGH@BCL2/t(14;18)(q32;q21): an aggressive disease with heterogeneous histology, germinal center B-cell immunophenotype and poor outcome. Mod Pathol, 2011; 25: 145–156.
Johnson NA, Savage KJ, Ludkovski O, Ben-Neriah S, Woods R, Steidl C et al. Lymphomas with concurrent BCL2 and MYC translocations: the critical factors associated with survival. Blood, 2009; 114: 2273–2279.
Schmitt CA, Lowe SW Bcl-2 mediates chemoresistance in matched pairs of primary E(mu)-myc lymphomas in vivo. Blood Cells Molecules Dis, 2001; 27: 206–216.
Vaux DL, Cory S, Adams JM Bcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells. Nature, 1988; 335: 440–442.
Strasser A, Harris AW, Bath ML, Cory S Novel primitive lymphoid tumours induced in transgenic mice by cooperation between myc and bcl-2. Nature, 1990; 348: 331–333.
Wu D, Wood BL, Dorer R, Fromm JR ‘Double-Hit’ mature B-cell lymphomas show a common immunophenotype by flow cytometry that includes decreased CD20 expression. Am Journal Clin Pathol, 2010; 134: 258–265.
Harrington AM, Olteanu H, Kroft SH, Eshoa C The unique immunophenotype of double-hit lymphomas. Am J Clin Pathol, 2011; 135: 649–650.
Hu Y, Turner MJ, Shields J, Gale MS, Hutto E, Roberts BL et al. Investigation of the mechanism of action of alemtuzumab in a human CD52 transgenic mouse model. Immunology, 2009; 128: 260–270.
Barabe F, Kennedy JA, Hope KJ, Dick JE Modeling the initiation and progression of human acute leukemia in mice. Science, 2007; 316: 600–604.
Bhatia K, Huppi K, Spangler G, Siwarski D, Iyer R, Magrath I Point mutations in the c-Myc transactivation domain are common in Burkitt's lymphoma and mouse plasmacytomas. Nat Genet, 1993; 5: 56–61.
Smith-Sorensen B, Hijmans EM, Beijersbergen RL, Bernards R Functional analysis of Burkitt's lymphoma mutant c-Myc proteins. J Biol Chem, 1996; 271: 5513–5518.
Gu W, Bhatia K, Magrath IT, Dang CV, Dalla-Favera R Binding and suppression of the Myc transcriptional activation domain by p107. Science, 1994; 264: 251–254.
Moreau T, Bardin F, Imbert J, Chabannon C, Tonnelle C Restriction of transgene expression to the B-lymphoid progeny of human lentivirally transduced CD34+ cells. Mol Ther, 2004; 10: 45–56.
Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO A system for Cre-regulated RNA interference in vivo. Proc Natl Acad Sci USA, 2008; 105: 13895–13900.
Zhang CC, Kaba M, Iizuka S, Huynh H, Lodish HF Angiopoietin-like 5 and IGFBP2 stimulate ex vivo expansion of human cord blood hematopoietic stem cells as assayed by NOD/SCID transplantation. Blood, 2008; 111: 3415–3423.
Giassi LJ, Pearson T, Shultz LD, Laning J, Biber K, Kraus M et al. Expanded CD34+ human umbilical cord blood cells generate multiple lymphohematopoietic lineages in NOD-scid IL2rgamma(null) mice. Exp Biol Med (Maywood), 2008; 233: 997–1012.
Shultz LD, Lyons BL, Burzenski LM, Gott B, Chen X, Chaleff S et al. Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2R gamma null mice engrafted with mobilized human hemopoietic stem cells. J Immunol, 2005; 174: 6477–6489.
Kelly PN, Puthalakath H, Adams JM, Strasser A Endogenous bcl-2 is not required for the development of Emu-myc-induced B-cell lymphoma. Blood, 2007; 109: 4907–4913.
Prendergast GC Mechanisms of apoptosis by c-Myc. Oncogene, 1999; 18: 2967–2987.
McDonnell TJ, Deane N, Platt FM, Nunez G, Jaeger U, McKearn JP et al. bcl-2-immunoglobulin transgenic mice demonstrate extended B cell survival and follicular lymphoproliferation. Cell, 1989; 57: 79–88.
Rangarajan A, Hong SJ, Gifford A, Weinberg RA Species- and cell type-specific requirements for cellular transformation. Cancer cell, 2004; 6: 171–183.
Chung KY, Morrone G, Schuringa JJ, Plasilova M, Shieh JH, Zhang Y et al. Enforced expression of NUP98-HOXA9 in human CD34(+) cells enhances stem cell proliferation. Cancer Res, 2006; 66: 11781–11791.
Wunderlich M, Krejci O, Wei J, Mulloy JC Human CD34+ cells expressing the inv(16) fusion protein exhibit a myelomonocytic phenotype with greatly enhanced proliferative ability. Blood, 2006; 108: 1690–1697.
Buske C, Feuring-Buske M, Antonchuk J, Rosten P, Hogge DE, Eaves CJ et al. Overexpression of HOXA10 perturbs human lymphomyelopoiesis in vitro and in vivo. Blood, 2001; 97: 2286–2292.
Chung KY, Morrone G, Schuringa JJ, Wong B, Dorn DC, Moore MA Enforced expression of an Flt3 internal tandem duplication in human CD34+ cells confers properties of self-renewal and enhanced erythropoiesis. Blood, 2005; 105: 77–84.
Schuringa JJ, Chung KY, Morrone G, Moore MA Constitutive activation of STAT5A promotes human hematopoietic stem cell self-renewal and erythroid differentiation. J Exp Med, 2004; 200: 623–635.
Montes R, Ayllon V, Gutierrez-Aranda I, Prat I, Hernandez-Lamas MC, Ponce L et al. Enforced expression of MLL-AF4 fusion in cord blood CD34+ cells enhances the hematopoietic repopulating cell function and clonogenic potential but is not sufficient to initiate leukemia. Blood, 2011; 117: 4746–4758.
Basecke J, Schwieger M, Griesinger F, Schiedlmeier B, Wulf G, Trumper L et al. AML1/ETO promotes the maintenance of early hematopoietic progenitors in NOD/SCID mice but does not abrogate their lineage specific differentiation. Leuk Lymphoma, 2005; 46: 265–272.
Wendtner CM, Ritgen M, Schweighofer CD, Fingerle-Rowson G, Campe H, Jager G et al. Consolidation with alemtuzumab in patients with chronic lymphocytic leukemia (CLL) in first remission—experience on safety and efficacy within a randomized multicenter phase III trial of the German CLL Study Group (GCLLSG). Leukemia, 2004; 18: 1093–1101.
Gallamini A, Zaja F, Patti C, Billio A, Specchia MR, Tucci A et al. Alemtuzumab (Campath-1H) and CHOP chemotherapy as first-line treatment of peripheral T-cell lymphoma: results of a GITIL (Gruppo Italiano Terapie Innovative nei Linfomi) prospective multicenter trial. Blood, 2007; 110: 2316–2323.
Acknowledgements
We thank P Bak and Herman Eisen for helpful discussions and the Swanson Biotechnology Center for excellent technical support. This work was partly supported by grants from the Marble Family Foundation (to JC and MTH), Singapore-MIT Alliance for Research and Technology (to JC) and the NIH (R01-CA128803 to MTH). CPP is supported by a research fellowship of the German Research foundation. IL was supported in part by the MIT School of Science Cancer Research Fellowship, the Ludwig Academic Graduate Fellowship and the Medical Scientist Training Program (Grant Number T32GM007753 from the National Institute Of General Medical Sciences). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute Of General Medical Sciences or the National Institutes of Health.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Competing interests
The authors declare no conflicts of interest.
Additional information
Supplementary Information accompanies the paper on the Oncogene website
Supplementary information
Rights and permissions
About this article
Cite this article
Leskov, I., Pallasch, C., Drake, A. et al. Rapid generation of human B-cell lymphomas via combined expression of Myc and Bcl2 and their use as a preclinical model for biological therapies. Oncogene 32, 1066–1072 (2013). https://doi.org/10.1038/onc.2012.117
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/onc.2012.117
Keywords
This article is cited by
-
Differential impact of high-salt levels in vitro and in vivo on macrophage core functions
Molecular Biology Reports (2024)
-
Generation of a CRISPR activation mouse that enables modelling of aggressive lymphoma and interrogation of venetoclax resistance
Nature Communications (2022)
-
CAR-T cells targeting a nucleophosmin neoepitope exhibit potent specific activity in mouse models of acute myeloid leukaemia
Nature Biomedical Engineering (2020)
-
B-cell lymphomas with concurrent MYC and BCL2 abnormalities other than translocations behave similarly to MYC/BCL2 double-hit lymphomas
Modern Pathology (2015)
-
Unusual concurrent presentation of a double hit (MYC/BCL2) and follicular lymphoma in a young patient; case report and review of key recent developments in double-hit lymphomas
Journal of Hematopathology (2014)