Case–control study of metachronous tumor incidence in 76 patients with a single right-side CC who underwent curative surgery. Ten of them developed a right-side metachronous neoplasm in the follow-up period (6–100 months). Patients were stratified into two groups (high and low) according to the LINE-1 RDL in the NCM, using three different thresholds: RDL>4, RDL>5 and RDL>6 (a–c, respectively), and metachronous tumor incidence was studied using the log rank test. Threshold 5 represents approximately the median of RDL values in the entire patient sample. The P-values, the number of cases above and below the cutoff, as well as the sensitivity and specificity of the classification are indicated for every used cutoff. Cases with LINE-1 RDL higher than the stratification threshold (‘high risk’) are represented with a solid black line, while cases with LINE-1 RDL lower than the stratification threshold (‘low risk’) are represented by a dashed line. X-axis indicates the time after the surgical resection of the first lesion. Y-axis represents the proportion of patients who developed a second (metachronous) lesion in the right-side colon. Points in the curves correspond to censored cases. The results still retain statistically significance by applying Fisher's exact test (under the alternative hypothesis that true odds ratio is >1) with an event at 36 months set as the endpoint: P=0.05 for cutoff of 4; P=0.015 for the RDL cutoff of 5 and P=0.045 for cutoff of 6.