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A role for collagen XXIII in cancer cell adhesion, anchorage-independence and metastasis

Oncogene volume 31, pages 2362–2372 (2012)Cite this article

Abstract

Collagen XXIII is a transmembrane collagen previously shown to be upregulated in metastatic prostate cancer that has been used as a tissue and fluid biomarker for non-small cell lung cancer and prostate cancer. To determine whether collagen XXIII facilitates cancer cell metastasis in vivo and to establish a function for collagen XXIII in cancer progression, collagen XXIII knockdown cells were examined for alterations in in vivo metastasis as well as in vitro cell adhesion. In experimental and spontaneous xenograft models of metastasis, H460 cells expressing collagen XXIII shRNA formed fewer lung metastases than control cells. Loss of collagen XXIII in H460 cells also impaired cell adhesion, anchorage-independent growth and cell seeding to the lung, but did not affect cell proliferation. Corroborating a role for collagen XXIII in cell adhesion, overexpression of collagen XXIII in H1299 cells, which do not express endogenous collagen XXIII, enhanced cell adhesion. Consequent reduction in OB-cadherin, alpha-catenin, gamma-catenin, beta-catenin, vimentin and galectin-3 protein expression was also observed in response to loss of collagen XXIII. This study suggests a potential role for collagen XXIII in mediating metastasis by facilitating cell–cell and cell–matrix adhesion as well as anchorage-independent cell growth.

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Acknowledgements

We thank Dr Jenny Mu for her assistance with the survival surgery performed in our spontaneous model of metastasis experiment. We also thank Colin Tasi for his assistance with the western blots.

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Authors and Affiliations

  1. Department of Surgery, Vascular Biology Program, Karp Family Research Laboratories, Children's Hospital, Boston, MA, USA

    K A Spivey, I Chung, J Banyard, I Adini & B R Zetter

  2. Clinical Research Program, Children's Hospital Boston, Boston, MA, USA

    H A Feldman

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  1. K A Spivey
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  2. I Chung
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  3. J Banyard
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  4. I Adini
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  5. H A Feldman
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  6. B R Zetter
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Correspondence to B R Zetter.

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Competing interests

B Zetter is a consultant and has equity in Predictive Biosciences, which has licensed the rights to collagen XXIII when used as a cancer biomarker. K Spivey, I Chung, J. Banyard, I Adini and H Feldman declare no conflict of interest.

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Supplementary Information accompanies the paper on the Oncogene website

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Spivey, K., Chung, I., Banyard, J. et al. A role for collagen XXIII in cancer cell adhesion, anchorage-independence and metastasis. Oncogene 31, 2362–2372 (2012). https://doi.org/10.1038/onc.2011.406

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