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P2X7 receptor activation enhances SK3 channels- and cystein cathepsin-dependent cancer cells invasiveness

Oncogene volume 30, pages 2108–2122 (2011)Cite this article

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Abstract

ATP-gated P2X7 receptors (P2X7R) are unusual plasma membrane ion channels that have been extensively studied in immune cells. More recently, P2X7R have been described as potential cancer cell biomarkers. However, mechanistic links between P2X7R and cancer cell processes are unknown. Here, we show, in the highly aggressive human breast cancer cell line MDA-MB-435s, that P2X7 receptor is highly expressed and fully functional. Its activation is responsible for the extension of neurite-like cellular prolongations, of the increase in cell migration by 35% and in cell invasion through extracellular matrix by 150%. The change in cancer cell morphology and the increased migration appeared to be due to the activation of Ca2+-activated SK3 potassium channels. The enhanced invasion through the extracellular matrix was related to the increase of mature forms of cysteine cathepsins in the extracellular medium, which was independent of SK3 channel activity and not associated with cell death. Pharmacological targeting of P2X7R in vivo was crucial for cell invasiveness in a zebrafish model of metastases. Our results demonstrate a novel mechanistic link between P2X7R functionality in cancer cells and invasiveness, a key parameter in tumour growth and in the development of metastases. They also suggest a potential therapeutic role for the newly developed P2X7R antagonists.

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Acknowledgements

We thank Pr Jean-Christophe Pagès, Dr Christine Collin and Dr Virginie Joulin for the development of small hairpin RNA cell lines, Elizabeth Martin, Mari C. Baños, Encarnación Sánchez and Isabelle Domingo for technical assistance. We are grateful to Pr Gilles Lalmanach for helpful discussions and Pierre-Yves Sizaret for his help with confocal microscopy. We thank Dr Michael Jarvis (Abbott Laboratories, USA) for the generous gift of A740003. This work was supported by the Biotechnology and Biological Sciences Research Council, by a grant from the ‘Ligue Nationale Contre le Cancer—Région Centre’, the ‘Association CANCEN’, the ‘Ministère de la Recherche et des Technologies’, the ‘Institut National de la Santé et de la Recherche Médicale’ (INSERM) and the ‘Partenariat Hubert Curien—Alliance’ from the French Foreign and European Ministry and the French Embassy in the United Kingdom. A C was funded by the ‘Institut National du Cancer (INCa)’.

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Authors and Affiliations

  1. Inserm U921, Université François Rabelais de Tours, 10 Boulevard Tonnellé, Tours, France

    B Jelassi, A Chantôme & S Roger

  2. Telomerase, Cancer and Aging Group, University Hospital ‘Virgen de la Arrixaca’-FFIS, Carretera Palmar, Murcia, Spain

    F Alcaraz-Pérez & M L Cayuela

  3. Inflammation and Experimental Surgery Group, University Hospital ‘Virgen de la Arrixaca’-FFIS, Carretera Palmar, Murcia, Spain

    A Baroja-Mazo & P Pelegrin

  4. Faculty of Life Science, Michael Smith Building D3315, University of Manchester, Manchester, UK

    P Pelegrin & A Surprenant

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  1. B Jelassi
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Correspondence to S Roger.

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Jelassi, B., Chantôme, A., Alcaraz-Pérez, F. et al. P2X7 receptor activation enhances SK3 channels- and cystein cathepsin-dependent cancer cells invasiveness. Oncogene 30, 2108–2122 (2011). https://doi.org/10.1038/onc.2010.593

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