Effect of THCV on insulin-induced phosphorylation of Akt in insulin-resistant rat C2C12 myotubes. Differentiated C2C12 cells were rendered insulin-resistant following 24 h incubation with 0.25 mM palmitic acid (PA) and co-treated with either THCV, AM251 or vehicle, at the concentrations shown. (a) Representative western blot for insulin stimulation of Akt phosphorylation in insulin-resistant cells. (b) Densitometric quantification of n=2 separate western blots, indicating the lower stimulation by acute insulin of pAKT/AKT levels in desensitized cells as compared to insulin-sensitive cells (naïve), considered as 1. (c) Effect of THCV or AM251 on insulin-induced stimulation of pAKT/AKT levels in insulin-resistant cells as compared to insulin-resistant cells only treated with acute insulin and vehicle (DMSO), considered as 1. #P<0.05 vs naïve. *P<0.05 vs DMSO, as assessed by ANOVA followed by the Bonferroni’s test.