Table 1 Primers used for real-time RT-PCR

From: Yacon diet (Smallanthus sonchifolius, Asteraceae) improves hepatic insulin resistance via reducing Trb3 expression in Zucker fa/fa rats

Target sequence Primers Primer sequence Band size (bp)
Rat Pepck BC081900 Pepck-620-F 5'-CAGCATGCGGATCATGACACGGAT-3' 530
  Pepck-1149-R 5'-GATTTGTCTTCACTGAGGTGCCCG-3'  
Rat G6pase NM_013098 G6pase-525-F 5'-CGTCTTGTGGTTGGGATACTG-3' 164
  G6pase-688-R 5'-TCTGGAGGCTGGCATTGTAG-3'  
Rat Pgc-1α NM_031347 Pgc-1α-963-F 5'-CTCCTCATAAAGCCAACCAAG-3' 143
  Pgc-1α-1105-R 5'-TGTGCGTATAACTCAGATTGC-3'  
Rat Trb3 BC091120 Trb3-187-F 5′-GCTGCTTCTGCCGATGTTCC-3′ 209
  Trb3-395-R 5′-CAGTTGCCTTGCTCTCGTTCC-3′  
Cyclophilin A (Cph) BC106030 Cph-96-F 5'-CTCCTTTGAGCTGTTTGCAG-3' 325
  Cph-420-R 5'-CACCACATGCTTGCCAT-3'  
  1. Abbreviations: G6pase, glucose-6-phophatase; Pepck, phosphoenolpyruvate carboxykinase 1; RT-PCR, reverse transcription PCR.