Effect of yacon diet on Akt phosphorylation (Ser473) in skeletal muscle (a) and liver (b) samples. Basal and insulin clamp-stimulated skeletal muscle and liver samples of control group (□; basal: n=8, clamp: n=8) and yacon group (▪; basal: n=8, clamp: n=8) were obtained and homogenized as described in Methods. After fraction of the resulting homogenates by 10% SDS-polyacrylamide gel electrophoresis, proteins were transferred onto polyvinylidine difluoride membranes, Membranes were probed with phosphorylated Akt (Ser473) and Akt antibodies. The bar graphs show data quantification by NIH-image for results in skeletal muscle and liver. Values are means±s.e.m. of data obtained from the analysis of the rats fed with control or yacon. Data are expressed relative to basal control values. *: P<0.01 vs control rats.