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Structural basis for the coevolution of a viral RNA–protein complex

Nature Structural & Molecular Biology volume 15, pages 103105 (2008) | Download Citation

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Abstract

The cocrystal structure of the PP7 bacteriophage coat protein in complex with its translational operator identifies a distinct mode of sequence-specific RNA recognition when compared to the well-characterized MS2 coat protein–RNA complex. The structure reveals the molecular basis of the PP7 coat protein's ability to selectively bind its cognate RNA, and it demonstrates that the conserved β-sheet surface is a flexible architecture that can evolve to recognize diverse RNA hairpins.

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References

  1. 1.

    & Bacteriol. Rev. 36, 109–134 (1972).

  2. 2.

    , , , & Virology 206, 611–625 (1995).

  3. 3.

    , , & Biochemistry 22, 2601–2610 (1983).

  4. 4.

    , & J. Biol. Chem. 276, 22507–22513 (2001).

  5. 5.

    & Nucleic Acids Res. 25, 2808–2815 (1997).

  6. 6.

    , & J. Biol. Chem. 271, 31839–31845 (1996).

  7. 7.

    et al. Structure 14, 487–495 (2006).

  8. 8.

    , , & Nature 345, 36–41 (1990).

  9. 9.

    et al. Structure 3, 255–263 (1995).

  10. 10.

    , , , & Structure 4, 543–554 (1996).

  11. 11.

    , , & J. Mol. Biol. 271, 759–773 (1997).

  12. 12.

    & Nucleic Acids Res. 30, 4138–4144 (2002).

  13. 13.

    et al. J. Mol. Biol. 270, 724–738 (1997).

  14. 14.

    & Biochemistry 26, 8221–8227 (1987).

  15. 15.

    , , , & Nature 371, 623–626 (1994).

  16. 16.

    & Nucleic Acids Res. 18, 6587–6594 (1990).

  17. 17.

    & RNA 13, 868–880 (2007).

  18. 18.

    , & Cell 103, 1121–1131 (2000).

  19. 19.

    et al. Mol. Cell 2, 437–445 (1998).

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Acknowledgements

This work was supported by the US National Institutes of Health (grants AR-41480 and EB-002060 to R.H.S and National Research Service Award institutional training grant (5T32HL007675-19) support to J.A.C.) and the Albert Einstein Cancer Center. The authors wish to thank the staff at the National Synchrotron Light Source X29a beamline for assistance with data collection, the staff at the Argonne Advanced Photon Source Structural GenomiX Collaborative Access Team beamline for express crystallography data collection and G. Arenas, M. Hennig, U. Meier, S. Nguyen and S. Ryder for helpful discussions.

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Affiliations

  1. Departments of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, New York 10461, USA.

    • Jeffrey A Chao
    •  & Robert H Singer
  2. Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, New York 10461, USA.

    • Yury Patskovsky
    •  & Steven C Almo

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Contributions

J.A.C. designed and performed the experiments. Y.P. assisted with crystallography. J.A.C., Y.P., S.C.A. and R.H.S. wrote and discussed the manuscript.

Corresponding author

Correspondence to Robert H Singer.

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    Supplementary Text and Figures

    Supplementary Figures 1–3, Supplementary Table 1 and Supplementary Methods

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DOI

https://doi.org/10.1038/nsmb1327

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