RNA interference: unraveling a mystery

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Andrew Fire and Craig Mello have won the Nobel Prize in Medicine or Physiology for their discovery of RNA interference. Mary K. Montgomery, then a postdoc in the Fire laboratory, participated in some of the key experiments.

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References

  1. 1

    Fire, A. et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806–811 (1998).

  2. 2

    Fire, A., Albertson, D., Harrison, S. & Moerman, D. Production of antisense RNA leads to effective and specific inhibition of gene expression in C. elegans muscle. Development 113, 503–514 (1991).

  3. 3

    Izant, J.G. & Weintraub, H. Inhibition of thymidine kinase gene expression by antisense RNA: a molecular approach to genetic analysis. Cell 36, 1007–1015 (1984).

  4. 4

    Guo, S. & Kemphues, K. par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed. Cell 81, 611–620 (1995).

  5. 5

    May, R.C. & Plasterk, R.H. RNA interference spreading in C. elegans. Methods Enzymol. 392, 308–315 (2005).

  6. 6

    Mello, C. & Fire, A. DNA transformation. Methods Cell Biol. 48, 451–482 (1995).

  7. 7

    Seydoux, G. et al. Repression of gene expression in the embryonic germ lineage of C. elegans. Nature 382, 713–716 (1996).

  8. 8

    Montgomery, M.K., Xu, S. & Fire, A. RNA as a target of double-stranded RNA-mediated genetic interference in Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 95, 15502–15507 (1998).

  9. 9

    Napoli, C., Lemieux, C. & Jorgensen, R. Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppresion of homologous genes in trans. Plant Cell 2, 279–289 (1990).

  10. 10

    Romano, N. & Macino, G. Quelling: transient inactivation of gene expression in Neurospora crassa by transformation with homologous sequences. Mol. Microbiol. 6, 3343–3353 (1992).

  11. 11

    Grishok, A., Tabara, H. & Mello, C.C. Genetic requirements for inheritance of RNAi in C. elegans. Science 287, 2494–2497 (2000).

  12. 12

    Vastenhouw, N.L. et al. Gene expression: long-term gene silencing by RNAi. Nature 442, 882 (2006).

  13. 13

    Bernstein, E. & Allis, C.D. RNA meets chromatin. Genes Dev. 19, 1635–1655 (2005).

  14. 14

    Mette, M.F., Aufsatz, W., van der Winden, J., Matzke, M.A. & Matzke, A.J. Transcriptional silencing and promoter methylation triggered by double-stranded RNA. EMBO J. 19, 5194–5204 (2000).

  15. 15

    Qi, Y. et al. Distinct catalytic and non-catalytic roles of ARGONAUTE4 in RNA-directed DNA methylation. Nature 443, 1008–1012 (2006).

  16. 16

    Zamore, P.D., Tuschl, T., Sharp, P. & Bartel, D. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at the 21 to 23 nucleotide intervals. Cell 101, 25–33 (2000).

  17. 17

    Bernstein, E., Caudy, A.A., Hammond, S.M. & Hannon, G.J. Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409, 363–366 (2001).

  18. 18

    Sijen, T. et al. On the role of RNA amplification in dsRNA-triggered gene silencing. Cell 107, 465–476 (2001).

  19. 19

    Lee, R.C., Feinbaum, R.L. & Ambros, V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75, 843–854 (1993).

  20. 20

    Wightman, B., Ha, I. & Ruvkun, G. Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell 75, 855–862 (1993).

  21. 21

    Ketting, R.F., Haverkamp, T.H., van Luenen, H.G. & Plasterk, R.H. Mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNaseD. Cell 99, 133–141 (1999).

  22. 22

    Mourrain, P. et al. Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance. Cell 101, 533–542 (2000).

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