Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. Using a conditional mutant of the yeast H3-K4 methyltransferase, Set1p, we demonstrate rapid turnover of H3-K4me3 and H3-K4me2 in vivo and show this process requires Yjr119Cp, of the JARID1 family of JmjC proteins. Ectopic overexpression of mouse Jarid1B, a Yjr119Cp homolog, greatly diminished H3-K4me3 and H3-K4me2 in HeLa cells, suggesting these proteins function as K4 demethylases in vivo.
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We thank M. Cleary (University of Iowa), D. Turner (University of Michigan) and A. Varshavsky (California Institute of Technology) for reagents, D. Gottschling, N. Fong, K. Glover and B. Erickson for discussion and technical help, and the Cancer Center sequencing facility of the University of Colorado Health Sciences Center. This work was supported by US National Institutes of Health grants R01GM063873 and T32-GM08730 to D.L.B. and D.J.S. D.J.S. is a member of the Medical Scientist Training Program.
The authors declare no competing financial interests.
Genetic interaction between SET1 and YJR119C. (PDF 511 kb)
Loss of H3-K4me2/me3 in Hep3b cells overexpressing Jarid1B. (PDF 1738 kb)
CTD Ser2 phosphorylation in HeLa cells overexpressing Jarid1B. (PDF 818 kb)
CTD Ser5 phosphorylation in HeLa cells overexpressing Jarid1B. (PDF 1128 kb)
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Seward, D., Cubberley, G., Kim, S. et al. Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins. Nat Struct Mol Biol 14, 240–242 (2007) doi:10.1038/nsmb1200
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