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Structural basis of ubiquitin recognition by mammalian Eap45 GLUE domain

Nature Structural & Molecular Biology volume 13, pages 10311032 (2006) | Download Citation

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Abstract

ESCRT-II, a complex that sorts ubiquitinated membrane proteins to lysosomes, localizes to endosomes through interaction between the Vps36 subunit's GLUE domain and phosphatidylinositides (PIs). In yeast, a ubiquitin (Ub)-interacting NZF domain is inserted in Vps36 GLUE, whereas its mammalian counterpart, Eap45 GLUE, lacks the NZF domain. In the Eap45 GLUE–Ub complex structure, Ub binds far from the proposed PI-binding site of Eap45 GLUE, suggesting their independent binding.

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Protein Data Bank

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Protein Data Bank

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Acknowledgements

This work was supported in part by the Protein 3000 project, by grants-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan and by the Norwegian Cancer Society, the Research Council of Norway and the Novo-Nordisk Foundation.

Author information

Author notes

    • Satoshi Hirano

    Present address: Biometal Science Laboratory, RIKEN SPring-8 Center, Harima Institute, Sayo, Hyogo, 679-5148, Japan.

Affiliations

  1. Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), Tsukuba, Ibaraki 305–0801, Japan.

    • Satoshi Hirano
    • , Nobuhiro Suzuki
    • , Masato Kawasaki
    • , Daniel Trambaiolo
    • , Ryuichi Kato
    •  & Soichi Wakatsuki
  2. Department of Biochemistry, Faculty Division of the Norwegian Radium Hospital, University of Oslo, Montebello, N-0310 Oslo, Norway.

    • Thomas Slagsvold
    •  & Harald Stenmark

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Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Soichi Wakatsuki.

Supplementary information

PDF files

  1. 1.

    Supplementary Fig. 1

    Sequence alignment of the GLUE domains.

  2. 2.

    Supplementary Fig. 2

    Electrostatic surface potential of the phosphoinositide-binding site of GLUE domains.

  3. 3.

    Supplementary Fig. 3

    Comparison of the ubiquitin-binding sites of mouse Eap45 and yeast Vps36.

  4. 4.

    Supplementary Fig. 4

    Schematic drawing of ESCRT-II binding to ubiquitinated cargo and endosomal membrane.

  5. 5.

    Supplementary Table 1

    Data collection and refinement statistics.

  6. 6.

    Supplementary Table 2

    Sequences of QuikChange primers for Eap45 GLUE mutants.

  7. 7.

    Supplementary Methods

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DOI

https://doi.org/10.1038/nsmb1163

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