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Identification of the FtsK sequence-recognition domain

Nature Structural & Molecular Biology volume 13, pages 10231025 (2006) | Download Citation

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Abstract

FtsK is a prokaryotic multidomain DNA translocase that coordinates chromosome segregation and cell division. FtsK is membrane anchored at the division septum and, guided by highly skewed DNA sequences, translocates the chromosome to bring the terminus of replication to the septum. Here, we use in vitro single-molecule and ensemble methods to unveil a mechanism of action in which the translocation and sequence-recognition activities are performed by different domains in FtsK.

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Acknowledgements

We dedicate this work to our friend and colleague Nick Cozzarelli, who passed away during preparation of this paper. We thank J. Moffitt, J. Gore, P. Pease and N. Crisona for critiques. US National Institutes of Health Grant GM31655 (J.L.P. and N.R.C.) and the Human Frontiers Science Organization (M.N.) supported this work.

Author information

Affiliations

  1. Department of Molecular and Cell Biology University of California, Berkeley, California 94720-3204, USA.

    • Jerod L Ptacin
    • , Marcelo Nöllmann
    • , Carlos Bustamante
    •  & Nicholas R Cozzarelli
  2. Department of Physics, University of California, Berkeley, California 94720-3204, USA.

    • Carlos Bustamante
  3. In memoriam.

    • Nicholas R Cozzarelli

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Competing interests

The authors declare no competing financial interests.

Supplementary information

PDF files

  1. 1.

    Supplementary Fig. 1

    FtsK50CΔγ triplex displacement is not affected by FRS orientation.

  2. 2.

    Supplementary Fig. 2

    Burst size distributions.

  3. 3.

    Supplementary Fig. 3

    FtsK50C recognizes FRS, whereas FtsK50CΔγ does not.

  4. 4.

    Supplementary Data

  5. 5.

    Supplementary Methods

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DOI

https://doi.org/10.1038/nsmb1157

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