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'Screw-cap' clamp loader proteins that thread

Nature Structural & Molecular Biology volume 11, pages 580581 (2004) | Download Citation

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Structures of the clamp loader–clamp complex reveal that nature uses machined parts in the form of a spiral scaffold (the clamp loader) to thread a circlet (the clamp) on a helix of DNA. The scaffold is then replaced by an interpretive copying machine, the polymerase, to eventually generate the replisome for DNA replication.

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Affiliations

  1. Z.Z., M.M.S. and S.J.B. are in the Department of Chemistry, 415 Wartik Laboratory, The Pennsylvania State University, University Park, Pennsylvania 16802, USA. sjb1@psu.edu

    • Zhihao Zhuang
    • , Michelle M Spiering
    •  & Stephen J Benkovic
  2. A.J.B. is in the Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106, USA.

    • Anthony J Berdis
  3. M.A.T. is at the MRC Cancer Cell Unit, Hutchinson/MRC Research Centre, Hills Road, Cambridge CB2 2XZ, UK.

    • Michael A Trakselis

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DOI

https://doi.org/10.1038/nsmb0704-580

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