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Axonemal dyneins winch the cilium

In this issue, Ishikawa and colleagues provide direct evidence to support the 'winch' hypothesis for the dynein motor mechanism, in which the AAA domain motor unit is displaced parallel to the doublet microtubule long axis rather than undergoing a rotary motion.

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Figure 1: Axonemal dynein.
Figure 2: Volume rendering of tomograms of axonemes in the apo and ADP.Vi forms presented by Movassagh et al.1.

References

  1. 1

    Movassagh, T., Bui, K., Sakakibara, H., Oiwa, K. & Ishikawa, T. Nat. Struct. Mol. Biol. 17, 761–767 (2010).

    CAS  Article  PubMed  Google Scholar 

  2. 2

    Nicastro, D. et al. Science 313, 944–948 (2006).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  3. 3

    Bui, K.H., Sakakibara, H., Movassagh, T., Oiwa, K. & Ishikawa, T. J. Cell Biol. 183, 923–932 (2008).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  4. 4

    Sakakibara, H. & Nakayama, H. J. Cell Sci. 111, 1155–1164 (1998).

    CAS  PubMed  Google Scholar 

  5. 5

    Furuta, A., Yagi, T., Yanagisawa, H., Higuchi, H. & Kamiya, R. J. Biol. Chem. 284, 5927–5935 (2009).

    CAS  Article  PubMed  Google Scholar 

  6. 6

    Liu, Z. et al. Eukaryot. Cell 7, 1136–1145 (2008).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  7. 7

    Oda, T., Hirokawa, N. & Kikkawa, M. J. Cell Biol. 177, 243–252 (2007).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  8. 8

    King, S.M. & Kamiya, R. Axonemal dyneins: assembly, structure and force generation. in The Chlamydomonas Source Book 2nd edn, Vol. 3 (ed. Witman, G.B.) 131–208 (Elsevier, San Diego, USA 2008).

    Google Scholar 

  9. 9

    Burgess, S.A., Walker, M.L., Sakakibara, H., Knight, P.J. & Oiwa, K. Nature 421, 715–718 (2003).

    CAS  Article  Google Scholar 

  10. 10

    Roberts, A.J. et al. Cell 136, 485–495 (2009).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  11. 11

    Carter, A.P. et al. Science 322, 1691–1695 (2008).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  12. 12

    Ueno, H., Yasunaga, T., Shingyoji, C. & Hirose, K. Proc. Natl. Acad. Sci. USA 105, 19702–19707 (2008).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  13. 13

    Johnson, K.A. J. Biol. Chem. 258, 13825–13832 (1983).

    CAS  PubMed  Google Scholar 

  14. 14

    Wickstead, B. & Gull, K. Traffic 8, 1708–1721 (2007).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  15. 15

    Sakakibara, H., Mitchell, D.R. & Kamiya, R.A. J. Cell Biol. 113, 615–622 (1991).

    CAS  Article  PubMed  Google Scholar 

  16. 16

    Mocz, G. & Gibbons, I.R. Biochemistry 35, 9204–9211 (1996).

    CAS  Article  Google Scholar 

  17. 17

    Kagami, O. & Kamiya, R. J. Cell Sci. 103, 653–664 (1992).

    CAS  Google Scholar 

  18. 18

    Yagi, T. et al. J. Biol. Chem. 280, 41412–41420 (2005).

    CAS  Article  PubMed  Google Scholar 

  19. 19

    Sakato, M., Sakakibara, H. & King, S.M. Mol. Biol. Cell 18, 3620–3634 (2007).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  20. 20

    Harrison, A. et al. Cell Motil. Cytoskeleton 52, 131–143 (2002).

    CAS  Article  PubMed  Google Scholar 

  21. 21

    Wakabayashi, K. & King, S.M. J. Cell Biol. 173, 743–754 (2006).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  22. 22

    Yanagisawa, H.A. & Kamiya, R. Biochem. Biophys. Res. Commun. 288, 443–447 (2001).

    CAS  Article  PubMed  Google Scholar 

  23. 23

    Hook, P. et al. J. Biol. Chem. 280, 33045–33054 (2005).

    Article  PubMed  Google Scholar 

  24. 24

    Patel-King, R.S. & King, S.M. J. Cell Biol. 186, 283–295 (2009).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The King laboratory is supported by grant GM51293 from the US National Institutes of Health.

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Correspondence to Stephen M King.

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King, S. Axonemal dyneins winch the cilium. Nat Struct Mol Biol 17, 673–674 (2010). https://doi.org/10.1038/nsmb0610-673

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