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Two new crystal structures of ubiquitin (Ub) in complex with fragments of the endosomal proteins Rabex-5 and HRS show intriguing new modes of Ub binding and reveal that both fragments can bind two Ub molecules simultaneously.

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Figure 1: Crystal structures and key intermolecular contacts in the HRS– and Rabex-5–Ub complexes.

References

  1. Hershko, A. Cell Death Differ. 12, 1191–1197 (2005).

    Article  CAS  Google Scholar 

  2. Welchman, R.L., Gordon, C. & Mayer, R.J. Nat. Rev. Mol. Cell Biol. 6, 599–609 (2005).

    Article  CAS  Google Scholar 

  3. Hicke, L. & Dunn, R. Annu. Rev. Cell Dev. Biol. 19, 141–172 (2003).

    Article  CAS  Google Scholar 

  4. Urbe, S. Essays Biochem. 41, 81–98 (2005).

    Article  CAS  Google Scholar 

  5. Katzmann, D.J., Odorizzi, G. & Emr, S.D. Nat. Rev. Mol. Cell Biol. 3, 893–905 (2002).

    Article  CAS  Google Scholar 

  6. Hirano, S. et al. Nat. Struct. Mol. Biol. 13, 272–277 (2006).

    Article  CAS  Google Scholar 

  7. Lee, S. et al. Nat. Struct. Mol. Biol. 13, 264–271 (2006).

    Article  CAS  Google Scholar 

  8. Pickart, C.M. & Fushman, D. Curr. Opin. Chem. Biol. 8, 610–616 (2004).

    Article  CAS  Google Scholar 

  9. Hicke, L., Schubert, H.L. & Hill, C.P. Nat. Rev. Mol. Cell Biol. 6, 610–621 (2005).

    Article  CAS  Google Scholar 

  10. VanDemark, A.P., Hofmann, R.M., Tsui, C., Pickart, C.M. & Wolberger, C. Cell 105, 711–720 (2001).

    Article  CAS  Google Scholar 

  11. Sundquist, W.I. et al. Mol. Cell 13, 783–789 (2004).

    Article  CAS  Google Scholar 

  12. Hamilton, K.S. et al. Structure 9, 897–904 (2001).

    Article  CAS  Google Scholar 

  13. Teo, H., Veprintsev, D.B. & Williams, R.L. J. Biol. Chem. 279, 28689–28696 (2004).

    Article  CAS  Google Scholar 

  14. Kang, R.S. et al. Cell 113, 621–630 (2003).

    Article  CAS  Google Scholar 

  15. Prag, G. et al. Cell 113, 609–620 (2003).

    Article  CAS  Google Scholar 

  16. Raiborg, C. & Stenmark, H. Cell Struct. Funct. 27, 403–408 (2002).

    Article  CAS  Google Scholar 

  17. Mattera, R., Tsai, Y.C., Weissman, A.M. & Bonifacino, J.S. J Biol. Chem., published online 5 January 2006 (10.1074/jbc.M509939200).

  18. Young, P., Deveraux, Q., Beal, R.E., Pickart, C.M. & Rechsteiner, M. J. Biol. Chem. 273, 5461–5467 (1998).

    Article  CAS  Google Scholar 

  19. Hofmann, K. & Falquet, L. Trends Biochem. Sci. 26, 347–350 (2001).

    Article  CAS  Google Scholar 

  20. Mueller, T.D. & Feigon, J. EMBO J. 22, 4634–4645 (2003).

    Article  CAS  Google Scholar 

  21. Swanson, K.A., Kang, R.S., Stamenova, S.D., Hicke, L. & Radhakrishnan, I. EMBO J. 22, 4597–4606 (2003).

    Article  CAS  Google Scholar 

  22. Wertz, I.E. et al. Nature 430, 694–699 (2004).

    Article  CAS  Google Scholar 

  23. Sloper-Mould, K.E., Jemc, J.C., Pickart, C.M. & Hicke, L. J. Biol. Chem. 276, 30483–30489 (2001).

    Article  CAS  Google Scholar 

  24. Prag, G. et al. Proc. Natl Acad. Sci. USA 102, 2334–2339 (2005).

    Article  CAS  Google Scholar 

  25. Akutsu, M. et al. FEBS Lett. 579, 5385–5391 (2005).

    Article  CAS  Google Scholar 

  26. Kawasaki, M. et al. Genes Cells 10, 639–654 (2005).

    Article  CAS  Google Scholar 

  27. Slagsvold, T. et al. J. Biol. Chem. 280, 19600–19606 (2005).

    Article  CAS  Google Scholar 

  28. Lim, W.A. Curr. Opin. Struct. Biol. 12, 61–68 (2002).

    Article  CAS  Google Scholar 

Download references

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Alam, S., Sundquist, W. U2. Nat Struct Mol Biol 13, 186–188 (2006). https://doi.org/10.1038/nsmb0306-186

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