Supplementary Figure 4 : MIWI2 retains partial nuclear localization in the absence of MILI.

From: A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming

Supplementary Figure 4

(a & c) Immunofluorescence with anti-MIWI2 antibody (a) and anti-HA antibody (c) on fetal testis of indicated genotypes with a seminiferous tubule shown. (b & d) Quantification of anti-MIWI2 (b) and anti-HA (d) fluorescence signal in the nucleus of indicated genotypes. 150 gonocytes from three animals, 100 and 97 gonocytes from two animals were analyzed in panel b from E16.5 Miwi2−/−, Miwi2+/− and Mili−/− fetal testes, respectively. 104, 104 and 103 gonocytes from two animals were analyzed in panel d from E16.5 Mili+/−, Mili+/−; Miwi2HA/+ and Mili−/−; Miwi2HA/+ fetal testes, respectively. Graphs show mean and s.d.