Supplementary Figure 3 : Transposon-related piRNAs in the absence of MILI.

From: A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming

Supplementary Figure 3

(a) Length distribution of small RNAs in wild-type (WT) and Mili−/− E16.5 fetal testis. (b) Quantification of LINE1 and IAP associated piRNAs in WT and Mili−/− E16.5 fetal testis. (c) piRNAs from WT and Mili−/− E16.5 fetal testis mapped to LINE1 and IAP consensus sequence allowing three mismatches. Positive (red) and negative (blue) values indicate sense and antisense piRNAs, respectively. (d) Ping-pong analysis of LINE1 and IAP associated piRNAs in WT and Mili−/− E16.5 fetal testis. The relative frequency of the distance between 5′ ends of complementary piRNAs for LINE1 (right) and IAP (left) is presented. (e) Percentage of LINE1 and IAP associated piRNAs in WT and Mili−/− E16.5 fetal testis with a U at the first position (1U) without an A at the position 10 and an A at position 10 (10A) without a U at position 1 is shown. (f) piRNAs from WT and Mili−/− E16.5 fetal testis mapped to loci whose methylation is dependent upon MIWI2 and MILI, respectively. Positive (red) and negative (blue) values indicate sense and antisense piRNAs, respectively. (g) Quantification of all or uniquely mapping piRNA densities across MILI and MIWI2-dependent loci is presented. Significance in e and g was assessed using BootstRatio algorithm, * indicates a p value of < 0.05, ** indicates a p value of < 0.005, *** indicates a p value of < 0.0005. Graphs in a, c, d, f show averages of values obtained from two animals. Individual data points in b, e, g show values obtained from two animals.