Supplementary Figure 6: Repair kinetics in cells synchronized in G1, analyzed by BLESS. | Nature Structural & Molecular Biology

Supplementary Figure 6: Repair kinetics in cells synchronized in G1, analyzed by BLESS.

From: Genome-wide mapping of long-range contacts unveils clustering of DNA double-strand breaks at damaged active genes

Supplementary Figure 6

a. Experimental pipeline used for Fig.5b: DIvA cells were synchronized using double thymidine block. 12h after release (entering in G1) cells were treated 4hours with 4OHT to induce DSBs, and further treated with auxin (IAA) for 2 additional hours to induce enzyme degradation and repair. Cells were collected before 4OHT treatment, 4h after 4OHT and 2h after IAA addition and subjected to BLESS. b. FACS analysis of the cell cycle at the different time points used for BLESS. c. Example of BLESS data obtained at a specific AsiSI induced DSB. DSB is indicated by an arrow d. Box plot showing the average BLESS count on +/- 500bp centered on the 100 DSBs analyzed in this study (top panel) or around the other AsiSI sites on the genome (bottom panel). Center line: median; Box limits: 2nd and 3rd quartiles; Whiskers: Maximum and minimum without outliers; Points: outliers.

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