Supplementary Figure 4: DSB clustering is favored at HR-prone DSBs. | Nature Structural & Molecular Biology

Supplementary Figure 4: DSB clustering is favored at HR-prone DSBs.

From: Genome-wide mapping of long-range contacts unveils clustering of DNA double-strand breaks at damaged active genes

Supplementary Figure 4

a. Clustering ability correlates with RAD51 binding. Number of interactions between each domain were measured and p values between damaged and undamaged samples were computed based on both replicates. –log10(p) are indicated, with negative fold changes (FC<0, damaged<undamaged) in blue, and positive fold change (FC>0, damaged>undamaged) in yellow. DSBs are sorted based on the ChIP-Seq level of RAD51 recruited on the break (Aymard, F. et al, Nat Struct Mol Biol. 4, 366-74, 2014). b. Cleavage efficiency is not significantly different between HR-prone and NHEJ-prone DSBs. Cleavage was measured by BLESS (Crosetto, N. et al, Nat Methods. 4, 362-5, 2013), in DIvA cells at HR-prone or NHEJ-prone DSBs (as indicated) (Clouaire, T. et al, manuscript in preparation). p=0.831 (Wilcoxon-Mann-Whitney test). Center line: median; Box limits: 2nd and 3rd quartiles; Whiskers: Maximum and minimum without outliers; Points: outliers.

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