Ribosome profiling provides a snapshot of the mRNA positions of all elongating ribosomes in the cell. A new powerful enhancement of the technique, translation complex profile sequencing (TCP–seq), extends this mapping to scanning ribosomal complexes. In addition to its usefulness as a tool for studying the regulation of translation initiation, TCP–seq provides specific and powerful signatures of bona fide translation.
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Baranov, P., Loughran, G. Catch me if you can: trapping scanning ribosomes in their footsteps. Nat Struct Mol Biol 23, 703–704 (2016). https://doi.org/10.1038/nsmb.3256
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DOI: https://doi.org/10.1038/nsmb.3256
