Classical PUF proteins bind to single-stranded RNA with sequence specificity that can be engineered by site-directed mutagenesis according to a simple RNA-recognition code. Now in-depth probing of the PUF RNA-recognition code enhances future design of PUF proteins and exposes hidden complexity in generating specificity.
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References
Wharton, R.P. & Aggarwal, A.K. Sci. STKE 2006, pe37 (2006).
Wickens, M., Bernstein, D.S., Kimble, J. & Parker, R. Trends Genet. 18, 150–157 (2002).
Campbell, Z.T., Valley, C.T. & Wickens, M. Nat. Struct. Mol. Biol. 21, 732–738 (2014).
Wang, X., McLachlan, J., Zamore, P.D. & Hall, T.M.T. Cell 110, 501–512 (2002).
Dong, S. et al. J. Biol. Chem. 286, 26732–26742 (2011).
Filipovska, A., Razif, M.F., Nygard, K.K. & Rackham, O. Nat. Chem. Biol. 7, 425–427 (2011).
Cheong, C.G. & Hall, T.M.T. Proc. Natl. Acad. Sci. USA 103, 13635–13639 (2006).
Choudhury, R., Tsai, Y.S., Dominguez, D., Wang, Y. & Wang, Z. Nat. Commun. 3, 1147 (2012).
Cooke, A., Prigge, A., Opperman, L. & Wickens, M. Proc. Natl. Acad. Sci. USA 108, 15870–15875 (2011).
Ozawa, T., Natori, Y., Sato, M. & Umezawa, Y. Nat. Methods 4, 413–419 (2007).
Tilsner, J. et al. Plant J. 57, 758–770 (2009).
Wang, Y., Cheong, C.G., Hall, T.M.T. & Wang, Z. Nat. Methods 6, 825–830 (2009).
Gerber, A.P., Herschlag, D. & Brown, P.O. PLoS Biol. 2, E79 (2004).
Opperman, L., Hook, B., DeFino, M., Bernstein, D.S. & Wickens, M. Nat. Struct. Mol. Biol. 12, 945–951 (2005).
Miller, M.T., Higgin, J.J. & Hall, T.M.T. Nat. Struct. Mol. Biol. 15, 397–402 (2008).
Wang, Y., Opperman, L., Wickens, M. & Hall, T.M.T. Proc. Natl. Acad. Sci. USA 106, 20186–20191 (2009).
Campbell, Z.T. et al. Cell Reports 1, 570–581 (2012).
Zhu, D., Stumpf, C.R., Krahn, J.M., Wickens, M. & Hall, T.M.T. Proc. Natl. Acad. Sci. USA 106, 20192–20197 (2009).
Qiu, C. et al. J. Biol. Chem. 287, 6949–6957 (2012).
Koh, Y.Y. et al. RNA 17, 718–727 (2011).
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Hall, T. Expanding the RNA-recognition code of PUF proteins. Nat Struct Mol Biol 21, 653–655 (2014). https://doi.org/10.1038/nsmb.2863
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DOI: https://doi.org/10.1038/nsmb.2863
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