(a,b) Interaction between GFP-tagged CNOT2 (full-length or fragments) and HA-tagged CNOT1 (a) and CNOT3 (b). GFP-tagged MBP served as a negative control. In all panels, cell lysates were treated with RNase A. (c,d) Interaction between GFP-tagged CNOT3 (full-length or fragments) and HA-tagged CNOT2 (c) and CNOT1 (d). (e,f) Interaction between GFP-tagged CNOT1 (full-length or fragments) and HA-tagged CNOT2 (e) and CNOT3 (f). In panel (f), the interaction with CNOT3 was analyzed in the absence (lanes 2 and 6) or presence (lanes 4 and 8) of CNOT2. (g) Interaction between MBP-CNOT2 fragments and His6-tagged CNOT1 (residues 1565–2371). (h) Interaction between MBP-CNOT2 fragments and GST-CNOT3 (residues 589–753). (i) Interaction between MBP-CNOT2 (residues 344–540) and GST-CNOT3 fragments. (j) Interaction between MBP-CNOT1 (residues 1595–2376) and GST-CNOT2 (residues 344–540) or GST-CNOT3 (residues 589–753).