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Coordinating the impact of structural genomics on the human α-helical transmembrane proteome

Nature Structural & Molecular Biology volume 20, pages 135–138 (2013)Cite this article

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Given the recent successes in determining membrane-protein structures, we explore the tractability of determining representatives for the entire human membrane proteome. This proteome contains 2,925 unique integral α-helical transmembrane-domain sequences that cluster into 1,201 families sharing more than 25% sequence identity. Structures of 100 optimally selected targets would increase the fraction of modelable human α-helical transmembrane domains from 26% to 58%, providing structure and function information not otherwise available.

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Figure 1: Flowchart of the analysis (Supplementary Notes 1 and 2).

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Acknowledgements

We thank I. Wilson, H. Berman, J. Chin and P. Preusch for critical comments on the manuscript. Research was supported by the US National Institutes of Health PSI:Biology grants U54 GM094662 (A.S., U.P.), U54 GM094618 (R.C.S.), U54 GM094625 (R.M.S., A.S., U.P.), U54 GM094584 (B.G.F.), U54 GM094599 (P.F.), U54 GM094611 (M.C.W., M.E.D., M.G.M.), U54 GM094610 (G.A.C., D.C.R., M.H.B.S.), U54 GM094608 (J.J.C.), U54 GM095315 (W.A.H., B.R., E.K.) and U54 GM094598 (D.L.S.).

Author information

Author notes

  1. Avner Schlessinger

    Present address: Present address: Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York, USA.,

Authors and Affiliations

  1. Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA

    Ursula Pieper, Avner Schlessinger & Andrej Sali

  2. Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA

    Ursula Pieper, Avner Schlessinger & Andrej Sali

  3. California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, California, USA

    Ursula Pieper, Avner Schlessinger & Andrej Sali

  4. Department of Bioinformatics and Computational Biology, Technical University Munich, Fakultät für Informatik, Garching, Germany

    Edda Kloppmann & Burkhard Rost

  5. Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, USA

    Geoffrey A Chang & Raymond C Stevens

  6. Harvard University Medical School, Boston, Massachusetts, USA

    James J Chou

  7. School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, New York, USA

    Mark E Dumont

  8. Transmembrane Protein Center, University of Wisconsin–Madison, Madison, Wisconsin, USA

    Brian G Fox

  9. Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, USA

    Petra Fromme

  10. New York Structural Biology Center, New York, New York, USA

    Wayne A Hendrickson & David L Stokes

  11. Hauptman Woodward Medical Research Institute, Buffalo, New York, USA

    Michael G Malkowski

  12. Division of Chemistry and Chemical Engineering, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, USA

    Douglas C Rees

  13. Molecular, Cellular and Developmental Biology, University of Colorado Boulder, Boulder, Colorado, USA

    Michael H B Stowell

  14. Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia, USA

    Michael C Wiener

  15. Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA

    Robert M Stroud

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Corresponding authors

Correspondence to Robert M Stroud, Raymond C Stevens or Andrej Sali.

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Supplementary information

Supplementary Figures, Tables and Notes

Supplementary Figures 1–4, Supplementary Tables 1–3 and Supplementary Notes 1–4 (PDF 1656 kb)

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Pieper, U., Schlessinger, A., Kloppmann, E. et al. Coordinating the impact of structural genomics on the human α-helical transmembrane proteome. Nat Struct Mol Biol 20, 135–138 (2013). https://doi.org/10.1038/nsmb.2508

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