Two-step insertion at the SecY translocon

Article metrics

SecY and Sec61 translocons mediate the orderly insertion of transmembrane segments into the lipid bilayer during membrane-protein biogenesis. Reporting in this issue, Ismail et al. now use a SecM-based molecular force sensor to show that the translocon exerts a pulling force on the nascent chain that is capable of mechanical action at two distinct stages of the insertion process.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Figure 1: Force measurement upon TMS insertion into the translocon.

References

  1. 1

    Rapoport, T.A. Nature 450, 663–669 (2007).

  2. 2

    Ismail, N., Hedman, R., Schiller, N. & von Heijne, G. Nat. Struct. Mol. Biol. 19, 1018–1022 (2012).

  3. 3

    Heinrich, S.U., Mothes, W., Brunner, J. & Rapoport, T. Cell 102, 233–244 (2000).

  4. 4

    Martoglio, B., Hofmann, M., Brunner, J. & Dobberstein, B. Cell 81, 207–214 (1995).

  5. 5

    Do, H., Falcone, D., Lin, J., Andrews, D. & Johnson, A. Cell 85, 369–378 (1996).

  6. 6

    Pitonzo, D., Yang, Z., Matsumura, Y., Johnson, A. & Skach, W. Mol. Biol. Cell 20, 685–698 (2009).

  7. 7

    McCormick, P.J., Miao, Y., Shao, Y., Lin, J. & Johnson, A. Mol. Cell 12, 329–341 (2003).

  8. 8

    Meacock, S.L., Lecomte, F., Crawshaw, S. & High, S. Mol. Biol. Cell 13, 4114–4129 (2002).

  9. 9

    Sadlish, H., Pitonzo, D., Johnson, A. & Skach, W. Nat. Struct. Mol. Biol. 12, 870–878 (2005).

  10. 10

    Butkus, M.E., Prundeanu, L.B. & Oliver, D.B. J. Bacteriol. 185, 6719–6722 (2003).

  11. 11

    Crowley, K.S., Liao, S., Worrell, V., Reinhart, G. & Johnson, A. Cell 78, 461–471 (1994).

  12. 12

    Junne, T., Kicik, L. & Spiess, M. Mol. Biol. Cell 21, 1662–1670 (2010).

  13. 13

    Devaraneni, P.K. et al. Cell 146, 134–147 (2011).

  14. 14

    Kida, Y., Morimoto, F. & Sakaguchi, M.J. J. Biol. Chem. 284, 2861–2866 (2009).

  15. 15

    Lu, Y. et al. J. Biol. Chem. 273, 568–576 (1998).

  16. 16

    Higy, M., Junne, T. & Spiess, M. Biochemistry 43, 12716–12722 (2004).

  17. 17

    Hessa, T. et al. Nature 450, 1026–1030 (2007).

  18. 18

    Skach, W.R. Nat. Struct. Mol. Biol. 16, 606–612 (2009).

Download references

Author information

Correspondence to William R Skach.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kim, S., Skach, W. Two-step insertion at the SecY translocon. Nat Struct Mol Biol 19, 975–977 (2012) doi:10.1038/nsmb.2402

Download citation

Further reading