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DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage

Nature Structural & Molecular Biology volume 19, pages 1093–1100 (2012)Cite this article

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Abstract

Ubiquitin-binding domains (UBDs) are crucial for recruiting many proteins to sites of DNA damage. Here we characterize C1orf124 (Spartan; referred to as DVC1), which has an UBZ4-type UBD found predominantly in DNA repair proteins. DVC1 associates with DNA replication factories and localizes to sites of DNA damage in human cells, in a manner that requires the ability of the DVC1 UBZ domain to bind to ubiquitin polymers in vitro and a conserved PCNA-interacting motif. DVC1 interacts with the p97 protein 'segregase'. We show that DVC1 recruits p97 to sites of DNA damage, where we propose that p97 facilitates the extraction of the translesion synthesis (TLS) polymerase (Pol) η during DNA repair to prevent excessive TLS and limit the incidence of mutations induced by DNA damage. We introduce DVC1 as a regulator of cellular responses to DNA damage that prevents mutations when DNA damage occurs.

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Figure 1: DVC1 localizes at DNA replication factories in S phase.
Figure 2: DVC1 localizes at replication factories and sites of DNA damage.
Figure 3: The UBZ domain of DVC1 binds to polyubiquitin chains.
Figure 4: Control of DVC1 localization by the UBZ domain and PIP motif.
Figure 5: DVC1 recruits p97-NPL4-UFD1 to sites of DNA damage.
Figure 6: Impact of DVC1 on retention of Pol η at sites of DNA damage.
Figure 7: Genotoxin hypersensitivity of DVC1-depleted cells.

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Acknowledgements

We thank A. Lehmann (University of Sussex) for Pol η expression plasmid, T. Shiomi (National Institute of Radiological Sciences, Japan) for HCT-116 RAD18−/− cells, M. Seidman (US National Institute on Aging, National Institutes of Health) for plasmid pSP189 and bacterial strain MBM7070, R. Toth (University of Dundee) for mutagenized DVC1 constructs, N. Mailand for communicating data before publication, and G. Alexandru and members of the Rouse laboratory for useful discussions. We thank the Medical Research Council Protein Phosphorylation Unit DNA Sequencing Service and the support teams of the Division of Signal Transduction Therapy, including N. Helps, J. Hastie and H. MacLauchlan, and members of the Centre for High Resolution Image Processing for help with microscopy and image processing. This study was funded by the Division of Signal Transduction Therapy Unit (funded by AstraZeneca, Boehringer-Ingelheim, GlaxoSmithKline, Merck KgaA, Janssen Pharmaceutica and Pfizer) associated with the Medical Research Council Protein Phosphorylation Unit, the Medical Research Council UK (E.J.D., C.L., T.J.M. and J.R.), the Association for International Cancer Research (E.J.D. and J.R.) and Cancer Research UK (P.A. and I.N.).

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  1. Emily J Davis and Christophe Lachaud: These authors contributed equally to this work.

Authors and Affiliations

  1. Medical Research Council Protein Phosphorylation Unit, Sir James Black Centre, University of Dundee, Dundee, Scotland, UK

    Emily J Davis, Christophe Lachaud, Thomas J Macartney & John Rouse

  2. Division of Cell and Developmental Biology, University of Dundee, Dundee, Scotland, UK

    Paul Appleton & Inke Näthke

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  1. Emily J Davis
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Contributions

J.R. designed the project, conceived the experiments and wrote the manuscript; E.J.D. and C.L. performed all of the experiments; T.J.M. made all of the plasmids used in this study; and P.A. and I.N. helped with microscopy.

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Correspondence to John Rouse.

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Davis, E., Lachaud, C., Appleton, P. et al. DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage. Nat Struct Mol Biol 19, 1093–1100 (2012). https://doi.org/10.1038/nsmb.2394

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