Abstract
Purified chromatin rings, excised from the PHO5 locus of Saccharomyces cerevisiae in transcriptionally repressed and activated states, were remodeled with RSC and ATP. Nucleosomes were translocated, and those originating on the promoter of repressed rings were removed, whereas those originating on the open reading frame (ORF) were retained. Treatment of the repressed rings with histone deacetylase diminished the removal of promoter nucleosomes. These findings point to a principle of promoter chromatin remodeling for transcription, namely that promoter specificity resides primarily in the nucleosomes rather than in the remodeling complex that acts upon them.
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Acknowledgements
This research was supported by US National Institutes of Health grant GM36659 (R.D.K).
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Y.L. designed, conducted and interpreted experiments and wrote the paper. J.G. and H.B. participated in the isolation of chromatin rings and in limit nuclease digestion experiments. B.M.-D. isolated chromatin rings and RSC. R.D.K. designed and interpreted experiments and wrote the paper.
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Lorch, Y., Griesenbeck, J., Boeger, H. et al. Selective removal of promoter nucleosomes by the RSC chromatin-remodeling complex. Nat Struct Mol Biol 18, 881–885 (2011). https://doi.org/10.1038/nsmb.2072
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DOI: https://doi.org/10.1038/nsmb.2072
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