Brief Communication | Published:

Homology-directed Fanconi anemia pathway cross-link repair is dependent on DNA replication

Nature Structural & Molecular Biology volume 18, pages 500503 (2011) | Download Citation

Abstract

Homologous recombination (also termed homology-directed repair, HDR) is a major pathway for the repair of DNA interstrand cross-links (ICLs) in mammalian cells. Cells from individuals with Fanconi anemia (FA) are characterized by extreme ICL sensitivity, but their reported defect in HDR is mild. Here we examined ICL-induced HDR using a GFP reporter and observed a profound defect in ICL-induced HDR in FA cells, but only when the reporter could replicate.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    Mutat. Res. 668, 4–10 (2009).

  2. 2.

    Nat. Rev. Genet. 8, 735–748 (2007).

  3. 3.

    et al. Cell 134, 969–980 (2008).

  4. 4.

    et al. Science 326, 1698–1701 (2009).

  5. 5.

    et al. Proc. Natl. Acad. Sci. USA 102, 1110–1115 (2005).

  6. 6.

    & Nat. Rev. Mol. Cell Biol. 11, 196–207 (2010).

  7. 7.

    , , & Genes Dev. 13, 2633–2638 (1999).

  8. 8.

    , , , & Mol. Cell. Biol. 16, 6820–6828 (1996).

  9. 9.

    & Mol. Carcinog. 48, 389–399 (2009).

  10. 10.

    Environ. Mol. Mutagen. 51, 527–539 (2010).

  11. 11.

    , , , & Nucleic Acids Res. 34, 4546–4553 (2006).

  12. 12.

    , & Nucleic Acids Res. 20, 4275–4281 (1992).

  13. 13.

    , , & Nucleic Acids Res. 33, 3492–3502 (2005).

  14. 14.

    , & Nucleic Acids Res. 36, 4680–4688 (2008).

  15. 15.

    , , & DNA Repair (Amst.) 6, 1670–1678 (2007).

  16. 16.

    et al. Mol. Cell. Biol. 22, 669–679 (2002).

  17. 17.

    et al. Proc. Natl. Acad. Sci. USA 103, 8768–8773 (2006).

  18. 18.

    , , , & Genes Dev. 15, 3237–3242 (2001).

  19. 19.

    & FEBS Lett. 584, 3703–3708 (2010).

  20. 20.

    et al. Mol. Cell 39, 25–35 (2010).

  21. 21.

    et al. Science 329, 219–223 (2010).

  22. 22.

    Environ. Mol. Mutagen. 51, 582–603 (2010).

  23. 23.

    , , , & Mol. Cell. Biol. 24, 9305–9316 (2004).

  24. 24.

    & Nat. Rev. Cancer 3, 23–34 (2003).

  25. 25.

    , & Mol. Cell. Biol. 5, 1822–1832 (1985).

  26. 26.

    , , , & Nucleic Acids Res. 32, 1143–1153 (2004).

  27. 27.

    et al. Mol. Cell 7, 249–262 (2001).

  28. 28.

    et al. Blood 100, 2414–2420 (2002).

  29. 29.

    & Plasmid 58, 1–12 (2007).

  30. 30.

    , , & Proc. Natl. Acad. Sci. USA 90, 7879–7883 (1993).

Download references

Acknowledgements

We thank K. Schlacher, J. LaRocque and L. Kass from the Jasin laboratory for comments on the manuscript. This work was supported by MFAG (My First AIRC Grant) 4765 from the Associazione Italiana per la Ricerca sul Cancro (M.B.), the Hecksher Foundation for Children (M.E.M.), the Byrne Fund (M.J.), and US National Institutes for Health grants P01CA94060 (M.E.M. and M.J.) and R01GM54668 (M.J.).

Author information

Affiliations

  1. Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.

    • Koji Nakanishi
    • , Erika Brunet
    •  & Maria Jasin
  2. Department of Public Health and Cell Biology, Section of Anatomy, University of Rome Tor Vergata, Rome, Italy.

    • Francesca Cavallo
    •  & Marco Barchi
  3. Muséum National d'Histoire Naturelle, Paris, France.

    • Loïc Perrouault
    • , Carine Giovannangeli
    •  & Erika Brunet
  4. Centre National de la Recherche Scientifique UMR7196, Paris, France.

    • Loïc Perrouault
    • , Carine Giovannangeli
    •  & Erika Brunet
  5. INSERM U565, Paris, France.

    • Loïc Perrouault
    • , Carine Giovannangeli
    •  & Erika Brunet
  6. Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.

    • Mary Ellen Moynahan

Authors

  1. Search for Koji Nakanishi in:

  2. Search for Francesca Cavallo in:

  3. Search for Loïc Perrouault in:

  4. Search for Carine Giovannangeli in:

  5. Search for Mary Ellen Moynahan in:

  6. Search for Marco Barchi in:

  7. Search for Erika Brunet in:

  8. Search for Maria Jasin in:

Contributions

K.N. designed and performed experiments, analyzed data and assisted in manuscript preparation; F.C. performed experiments and analyzed data; L.P. prepared the ICL-only substrates; C.G. supervised L.P. and assisted in manuscript preparation; M.E.M. provide supervision, analyzed data and assisted in manuscript preparation; M.B. supervised F.C. and contributed to data analysis; E.B. designed experiments and assisted in manuscript preparation; M.J. designed the experiments, supervised the project and wrote the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Maria Jasin.

Supplementary information

PDF files

  1. 1.

    Supplementary Text and Figures

    Supplementary Methods, Supplementary Figures 1–4 and Supplementary Table 1

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/nsmb.2029

Further reading