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Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae

Nature Structural & Molecular Biology volume 17, pages 244247 (2010) | Download Citation

Abstract

Nonsense-mediated decay (NMD) degrades mRNA containing premature translation termination codons. In yeast, NMD substrates are decapped and digested exonucleolytically from the 5′ end. Despite the requirement for translation in recognition, degradation of nonsense-containing mRNA is considered to occur in ribosome-free cytoplasmic P bodies. We show decapped nonsense-containing mRNA associate with polyribosomes, indicating that recognition and degradation are tightly coupled and that polyribosomes are major sites for degradation of aberrant mRNAs.

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Acknowledgements

The authors thank members of the Baker and Coller laboratories for critical evaluation of the manuscript. W.H. is supported by the American Heart Association. C.P. is supported by an undergraduate stipend provided by the US National Science Foundation. Funding was provided by the US National Institutes of Health (GM080465).

Author information

Affiliations

  1. Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio, USA.

    • Wenqian Hu
    • , Christine Petzold
    • , Jeff Coller
    •  & Kristian E Baker

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Contributions

W.H., J.C. and K.E.B. designed experimental strategies; W.H. and C.P. performed experimental analysis and interpreted data; J.C. and K.E.B. interpreted data and wrote the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Jeff Coller or Kristian E Baker.

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DOI

https://doi.org/10.1038/nsmb.1734

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