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Initial loss of secondary structure in the unfolding of barstar

Rapid (millisecond) far-UV circular dichroism and fluorescence-monitored kinetic studies of the unfolding of barstar demonstrate the accumulation of a compact intermediate devoid of much secondary structure, before the rate limiting step in unfolding.

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Nath, U., Agashe, V. & Udgaonkar, J. Initial loss of secondary structure in the unfolding of barstar. Nat Struct Mol Biol 3, 920–923 (1996). https://doi.org/10.1038/nsb1196-920

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