Rapid (millisecond) far-UV circular dichroism and fluorescence-monitored kinetic studies of the unfolding of barstar demonstrate the accumulation of a compact intermediate devoid of much secondary structure, before the rate limiting step in unfolding.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$189.00 per year
only $15.75 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Chan, H.S. & Dill, K.A. J. Chem. Phys. 100, 9238–9257 (1994).
Fersht, A.R. Curr. Opin. Struct. Biol. 5, 79–84 (1994).
Šali, A., Shakhnovich, E.I. & Karplus, M. Nature 369, 248–251 (1994).
Baldwin, R.L. Fold & Des. 1, R1–R8 (1996).
Schmid, F.X. in Protein Folding (ed. I.E. Creighton) 197–241 (W.H. Freeman and Co., New York, 1992).
Kiefhaber, T., Labhardt, A.M. & Baldwin, R.L. Nature 375, 513–515 (1995).
Hoeltzli, S.D. & Frieden, C. Proc. Natl. Acad. Sci. USA 92, 9318–9322 (1995).
Segawa, S. & Sugihara, M. Biopolymers 23, 2473–2488 (1984).
Kuwajima, K., Mitani, M. & Sugai, S. J. Molec. Biol. 206, 547–561 (1989).
Agashe, V.R., Shastry, M.C.R. & Udgaonkar, J.B. Nature 377, 754–757 (1995).
Schreiber, G. & Fersht, A.R. Biochemistry 32, 11195–11203 (1993).
Shastry, M.C.R., Agashe, V.R. & Udgaonkar, J.B. Protein Sci. 3, 1409–1417 (1994).
Shastry, M.C.R. & Udgaonkar, J.B. J. Molec. Biol. 247, 1013–1027 (1995).
Lubienski, M.J., Bycroft, M., Freund, S.M.V. & Fersht, A.R. Biochemistry 33, 8866–8877 (1994).
Kuwajima, K., Okayama, N., Yamamoto, K., Ishihara, T. & Sugai, S. FEBS Lett. 290, 135–138 (1991).
Buckle, A.M., Schreiber, G. & Fersht, A.R. Biochemistry 33, 8878–8889 (1994).
Cantor, C.R. & Schimmel, P.R. Biophys. Chem. vol. 2 (W.H. Freeman, San Francisco, 1980).
Ptitsyn, O.B. in Protein Folding (ed. I.E. Creighton) 243–300 (W.H. Freeman and Co., New York, 1992).
Shakhnovich, E.I. & Finkelstein, A.V. Biopolymers 28, 1667–1680 (1989).
Mark, A.E. & van Gunsteren, W. F. Biochemistry 31, 7745–7748 (1992).
Daggett, V. & Levitt, M. J. Molec. Biol. 232, 600–619 (1993).
Karplus, M. & Šali, A. Curr. Opin. Struct. Biol 5, 58–73 (1995).
Matouschek, A., Kellis, J.T., Serrano, L. & Fersht, A.R. Nature 342, 122–126 (1989).
Serrano, L., Matouschek, A. & Fersht, A.R. J. Molec. Biol. 224, 805–818 (1992).
Otzen, D.S., ElMasry, N., Jackson, S.E. & Fersht, A.R. Proc. Natl. Acad. Sci. USA 91, 10422–10425 (1994)
Caflisch, A. & Karplus, M. Proc. Natl. Acad. Sci. USA 91, 1746–1750 (1994).
Li, A. & Daggett, V. Proc. Natl. Acad. Sci. USA 91, 10430–10434 (1994).
Daggett, V., Li, A., Itzhaki, L.S., Otzen, D.E. & Fersht, A.R. J. Molec. Biol. 257, 430–440 (1996).
Robinson, D.R. & Jencks, W.P. J. Am. Chem. Soc. 87, 2462–2466 (1965).
Makhatadze, G.I. & Privalov, P.L. J. Molec. Biol. 226, 491–505 (1992).
Breslow, R. & Guo, T. Proc. Natl. Acad. Sci. USA 87, 167–169 (1990).
Swaminathan, R., Nath, U., Udgaonkar, J.B., Periasamy, N. & Krishnamoorthy, G. Biochemistry 35, 9150–9157 (1996).
Nath, U. & Udgaonkar, J.B. Biochemistry 34, 1702–1713 (1995).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nath, U., Agashe, V. & Udgaonkar, J. Initial loss of secondary structure in the unfolding of barstar. Nat Struct Mol Biol 3, 920–923 (1996). https://doi.org/10.1038/nsb1196-920
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/nsb1196-920
This article is cited by
-
Multiple intermediates and transition states during protein unfolding
Nature Structural Biology (1997)