Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

The structure of lactate dehydrogenase from Plasmodium falciparum reveals a new target for anti-malarial design

Nature Structural Biology volume 3, pages 912–915 (1996)Cite this article

The crystal structure of Plasmodium falciparum lactate dehydrogenase reveals a surprising shift in the position of the NADH cofactor that explains the unusual biochemical properties of this enzyme. There is also a distinctive surface cleft adjacent to the NADH binding pocket that forms an attractive target for inhibitor design.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

References

  1. Kell, A.J. Malaria (Oxford University Press, Oxford; 1991).

    Google Scholar 

  2. Royer, R.E., Deck, L.M., Campos, N.M., Hunsaker, L.A. & Vander Jagt, D.L. J. Med. Chem. 29, 1799–1801 (1986).

    Article  CAS  Google Scholar 

  3. Vander Jagt, D.L., Hunsaker, L.A. & Heidrich, J.E. Molec. & Biochem. Parasitology 4, 255–264 (1981).

    Article  CAS  Google Scholar 

  4. Dunn, C.R. et al. Philos. Trans. R. Soc. London, Sen B 332, 177–184 (1991).

    Article  CAS  Google Scholar 

  5. White, J.L. et al. J. Mol. Biol. 102, 759–779 (1976).

    Article  CAS  Google Scholar 

  6. Wigley, D.B. et al. J. Mol. Biol. 223, 317–335 (1992).

    Article  CAS  Google Scholar 

  7. Fromm, H.J. Biochim. Biophys. Acta 52, 199–200 (1961).

    Article  CAS  Google Scholar 

  8. Gutfreund, H., Cantwell, R., McMurray, C.H., Criddle, R.S. & Hathaway, G. Biochem. J. 106, 683–687 (1968).

    Article  CAS  Google Scholar 

  9. Coulson, C.J. & Rabin, B.R. FEBS Lett. 3, 333–334 (1969).

    Article  CAS  Google Scholar 

  10. Deng, H., Zheng, J., Clarke, A., Holbrook, J.J., Callender, R. & Burgner, J.W. Biochemistry 33, 2297–2305 (1994).

    Article  CAS  Google Scholar 

  11. Hewitt, C.O., Sessions, R.B. & Holbrook, J.J. Proceedings of the 5th European Meeting of Perspectives on Protein Engineering. Montepellier, France (1996).

    Google Scholar 

  12. Makler, M.T. & Hinrichs, D.J. Amer. J. Tropical Medicine & Hygiene 48, 205–210 (1993).

    Article  CAS  Google Scholar 

  13. Deck, L.M., Vander Jagt, D.L. & Royer, R.E. J. Med. Chem. 34, 3301–3305 (1991).

    Article  CAS  Google Scholar 

  14. Sessions, R.B., Dewar, V., Clarke, A.R. & Holbrook, J.J. Prot. Engng. (in the press).

  15. Wilks, H.M. et al. Biochemistry 31, 7802–7806 (1992).

    Article  CAS  Google Scholar 

  16. Collaborative Computing Project Number 4-Programs for Protein Crystallography. Acta Crystallogr. D50, 760–763 (1994).

  17. Jones, T.A., Zou, J.Y., Cowan, S.W., Kjeldgaard, M. Acta Crystallogr. A47, 110–119 (1991).

    Article  CAS  Google Scholar 

  18. Buünger, A.T. X-PLOR Manual Version 3.1 (Yale University: New Haven, CT, 1992).

    Google Scholar 

  19. Lamzin, V.S. & Wilson, K.S. Acta Cryst. D49, 129–147 (1993).

    CAS  Google Scholar 

  20. Nicholls, A., Sharp, K.A. & Honig, B. Proteins 11, 281–296 (1991).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Molecular Recognition Centre and Department of Biochemistry, University of Bristol School of Medical Sciences, Bristol, BS8 1TD, UK

    Cameron R. Dunn, Mark J. Banfield, John J. Barker, Christopher W. Higham, Kathleen M. Moreton, Dilek Turgut-Balik, R. Leo Brady & J. John Holbrook

Authors
  1. Cameron R. Dunn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mark J. Banfield
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. John J. Barker
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Christopher W. Higham
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kathleen M. Moreton
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Dilek Turgut-Balik
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. R. Leo Brady
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. J. John Holbrook
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dunn, C., Banfield, M., Barker, J. et al. The structure of lactate dehydrogenase from Plasmodium falciparum reveals a new target for anti-malarial design. Nat Struct Mol Biol 3, 912–915 (1996). https://doi.org/10.1038/nsb1196-912

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nsb1196-912

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing